Brassinosteroid enhanced the level of antioxidants under cadmium stress in Brassica juncea
Brassinosteroid enhanced the level of antioxidants under cadmium stress in Brassica juncea
复制标题
DOI：
10.1016/j.envexpbot.2006.06.002
复制
发表时间：
2007-05-01
影响因子：
5.7
通讯作者：
Ahmad, A.
中科院分区：
文献类型：
--
作者：
Hayat, S.;Ali, B.;Ahmad, A.
以60日龄芥菜型油菜（Brassica juncea）为试材，研究了镉和28-高油菜素（HBL）对芥菜型油菜生长、光合作用、碳酸酐酶、硝酸还原酶和抗氧化酶的影响。一周大的幼苗与营养液一起供应50、100或150 μ M的镉沿着。在第30天，将随后的幼苗用0.01 μ M的HBL喷洒到它们的叶子上。单独喂镉的植物表现出生长下降，碳酸酐酶（E.C. 4.2.1.1）和叶绿素色素以及净光合速率。硝酸盐含量、硝酸还原酶活性（E.C. 1.6.6.1随着营养液中镉浓度的增加，叶片和根中碳水化合物的含量均降低.与叶片相比，根中硝酸盐含量较高。然而，在硝酸还原酶和两种植物器官中的碳水化合物水平的情况下，这种趋势被逆转。在胁迫条件下，喷施HBL可克服镉的毒害作用，并能提高抗氧化酶[过氧化氢酶（E.C.）1.11.1.6）、过氧化物酶（E.C. 1.11.1.7）和超氧化物歧化酶（E.C.（1.15.1.1）]，脯氨酸含量均高于对照。他们的水平进一步增加，如果提供镉的植物也补充HBL，无论是在根和地上部分。但根中脯氨酸含量高于叶。(c)2006 Elsevier B. V.保留所有权利。
The change in plant growth, photosynthesis, carbonic anhydrase, nitrate reductase and antioxidative enzymes resulting from the feeding of cadmium and/or 28-homobrassinolide (HBL) to Brassica juncea were studied in 60-day-old plants. One-week old seedlings were supplied with 50, 100 or 150 mu M of cadmium along with the nutrient solution. Subsequent seedlings, at day 30, were sprayed with 0.01 mu M of HBL to their foliage. The plants fed with cadmium alone exhibited a decline in growth, the levels of carbonic anhydrase (E.C. 4.2.1.1) and chlorophyll pigments and net photosynthetic rate. Moreover, nitrate content, the activity of nitrate reductase (E.C. 1.6.6.1) and the level of carbohydrate, both in the leaves and roots, decreased as the concentration of cadmium, in nutrient solution, increased. Compared with the leaves, roots possessed a larger quantity of nitrate. However, the trend was reversed in case of nitrate reductase and the level of carbohydrates in the two plant organs. The toxic effect, generated by cadmium was overcome if the stressed plants were sprayed with HBL.The activities of antioxidative enzymes [viz. catalase (E.C. 1.11.1.6), peroxidase (E.C. 1.11.1.7) and superoxide dismutase (E.C. 1.15.1.1)] and the contents of proline increased, over the control, irrespective of the treatment. Their level increased further, if the plants supplied with cadmium were also supplemented with HBL, both in the roots and the aerial parts. Nevertheless, the contents of proline in roots were higher than the leaves. (c) 2006 Elsevier B.V. All rights reserved.