miR-455 Functions as a Tumor Suppressor Through Targeting GATA6 in Colorectal Cancer.
miR-455 Functions as a Tumor Suppressor Through Targeting GATA6 in Colorectal Cancer.
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DOI：
10.3727/096504018x15220579006875
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发表时间：
2019-02-21
影响因子：
3.1
通讯作者：
Caixia B
中科院分区：
文献类型：
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作者：
Yunqi H;Fangrui Y;Yongyan Y;Yunjian J;Wenhui Z;Kun C;Min L;Xianfeng L;Caixia B
新出现的证据表明，microRNAs（miRNAs）经常在人类癌症中异常表达。与此同时，miRNAs在调节多种细胞生物学过程中的重要性也得到了人们的重视。本研究的目的是探讨miR-455在调节结直肠癌（CRC）进展中的意义，并确定其可能的机制。我们发现miR-455在结直肠癌组织和细胞系中的表达显著降低。重要的是，miR-455的低表达与CRC患者的总体生存率差相关。miR-455在大肠癌细胞系中的过表达显著抑制细胞的体外增殖和迁移。此外，GATA结合蛋白6（GATA 6），其表达可以在CRC细胞系中被miR-455反向调节，被验证为miR-455的直接靶标。总体而言，我们的研究结果表明，miR-455作为肿瘤抑制因子发挥作用，其下调可能有助于CRC进展。本研究为今后大肠癌的治疗提供了新的靶点。
Emerging evidence indicates that microRNAs (miRNAs) are often aberrantly expressed in human cancers. Meanwhile, the importance of miRNAs in regulating multiple cellular biological processes has been appreciated. The aim of this study was to investigate the significance of miR-455 and identify its possible mechanism in regulating colorectal cancer (CRC) progression. We found that the expression of miR-455 was sharply reduced in CRC tissues and cell lines. Importantly, the low expression of miR-455 was associated with poor overall survival of CRC patients. Overexpression of miR-455 in CRC cell lines significantly inhibited cell proliferation and migration in vitro. Moreover, GATA-binding protein 6 (GATA6), whose expression can be inversely regulated by miR-455 in CRC cell lines, was validated as a direct target of miR-455. Overall, our results revealed that miR-455 functions as a tumor suppressor, and its downregulation may contribute to CRC progression. Our study may provide a novel therapeutic target for CRC in the future.