Aqueous normal-phase retention of nucleotides on silica hydride columns
Aqueous normal-phase retention of nucleotides on silica hydride columns
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DOI：
10.1016/j.chroma.2008.12.046
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发表时间：
2009-02-13
影响因子：
4.1
通讯作者：
Boysen, Reinhard I.
中科院分区：
文献类型：
--
作者：
Pesek, Joseph J.;Matyska, Maria T.;Boysen, Reinhard I.
研究了以氢化硅为基础的固定相用于核苷酸的保留和分析。测试了下面有氢化物表面的反相柱，以及未改性或最低限度改性的氢化物材料的反相柱。在这些体系中，水正相模式与高有机含量的流动相结合，并结合添加剂来控制pH值，以保留亲水核苷酸。等密度和梯度洗脱格式已用于优化分离的混合物含有多达七个成分。所开发的所有条件都适用于利用质谱检测的方法。(C) 2008 Elsevier B.V.版权所有
The use of silica hydride-based stationary phases for the retention and analysis of nucleotides has been investigated. Both reversed-phase columns with a hydride surface underneath as well as those with an unmodified or a minimally modified hydride material were tested. With these systems, an aqueous normal-phase mode was used with high organic content mobile phases in combination with an additive to control pH for the retention of the hydrophilic nucleotides. Isocratic and gradient elution formats have been used to optimize separations for mixtures containing up to seven components. All conditions developed are suitable for methods that utilize mass spectrometry detection. (C) 2008 Elsevier B.V. All rights reserved.