Enhanced osteogenic differentiation and biomineralization in mouse mesenchymal stromal cells on a β-TCP robocast scaffold modified with collagen nanofibers
Enhanced osteogenic differentiation and biomineralization in mouse mesenchymal stromal cells on a β-TCP robocast scaffold modified with collagen nanofibers
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DOI：
10.1039/c5ra26670j
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发表时间：
2016-02
期刊：
影响因子：
3.9
通讯作者：
Fen Zou;Naru Zhao;Xiaoling Fu;Jingjing Diao;Yijuan Ma;Xiaodong Cao;Shuangyan Wan;Shizheng Zhong;Yingjun Wang
中科院分区：
文献类型：
--
作者：
Fen Zou;Naru Zhao;Xiaoling Fu;Jingjing Diao;Yijuan Ma;Xiaodong Cao;Shuangyan Wan;Shizheng Zhong;Yingjun Wang
磷酸钙陶瓷作为骨移植材料已广泛应用于临床。然而，根据传统技术，难以产生具有定制的内部多孔结构的磷酸钙骨移植物，所述定制的内部多孔结构可以确保最佳的生物相容性和足够的机械性能。在这项研究中，β-TCP骨支架具有明确的相互连接的多孔结构的机器人铸造。为了更好地模拟骨的天然细胞外基质而不牺牲机械强度，将I型胶原凝胶涂覆在烧结的β-TCP支架的细丝上以形成与骨中的天然胶原纳米纤维相似的超细纤维网络。烧结的β-TCP支架和胶原凝胶涂层支架均支持小鼠间充质基质细胞（mMSCs）的生长。同时，β-TCP支架上的仿生胶原纳米纤维薄层通过上调ALP、Runx-2、I型胶原、OPN、BSP和BMP-2的表达，显著促进mMSCs向成骨细胞分化。此外，在胶原凝胶包被的支架上的mMSCs中诱导了更活跃的基质囊泡（MV）的生物发生，通过SEM和TEM结果以及基质囊泡组分的基因表达的显著增加来证明，这表明基质矿化的起始增强。我们的研究不仅说明了胶原凝胶涂层的β-TCP robocast支架用于骨修复的潜在用途，而且还强调了胶原纳米纤维在支架中的掺入的重要性。
Calcium phosphate ceramics have been widely used in clinics as bone grafts. However, according to traditional techniques, it is difficult to create calcium phosphate bone grafts with tailored internal porous structures that may ensure optimal biocompatibility and sufficient mechanical properties. In this study, β-TCP bone scaffolds with well-defined inter-connective porous structures were fabricated by robocasting. With the aim of better mimicking the native extracellular matrix of bone without sacrificing the mechanical strength, type I collagen gel was coated on the filaments of the sintered β-TCP scaffolds to form an ultrafine fibrous network that was similar to the natural collagen nanofibers in bone. Both sintered β-TCP scaffolds and collagen gel coated scaffolds supported the growth of mouse mesenchymal stromal cells (mMSCs). Meanwhile, the thin layer of biomimetic collagen nanofibers on the β-TCP scaffolds significantly stimulated the osteoblastic differentiation of mMSCs by up-regulating the expression of ALP, Runx-2, collagen I, OPN, BSP and BMP-2. Additionally, a more active biogenesis of matrix vesicles (MVs) was induced in the mMSCs on the collagen gel coated scaffolds, evidenced by SEM and TEM results together with a significant increase of the gene expression of matrix vesicle components, indicating an enhanced initiation of matrix mineralization. Our study not only illustrates the potential use of the collagen gel coated β-TCP robocast scaffold for bone repair but also highlights the significance of the incorporation of collagen nanofibers in the scaffolds.