What drives changes in the virulence and antibiotic resistance ofVibrio harveyiin the South China Sea?
What drives changes in the virulence and antibiotic resistance ofVibrio harveyiin the South China Sea?
复制标题
是什么推动了南海哈维氏弧菌毒力和抗生素耐药性的变化？
DOI：
10.1111/jfd.13197
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发表时间：
2020-06-18
影响因子：
2.5
通讯作者：
Feng, Juan
中科院分区：
文献类型：
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作者：
Deng, Yiqin;Xu, Liwen;Feng, Juan
为了解细菌毒力和耐药性变化的驱动环境因素，对我国南方沿海患病海水鱼中分离的哈维氏弧菌（Vibrio harveyi）的流行情况、耐药性以及耐药性和毒力基因进行了测定。我们隔离了2、52和53 V。用16 SrRNA-toxR（Vh）-rctB基因多位点序列分析对福建、海南和广东的3株harvey 1菌株进行了鉴定。9个典型毒力基因在海南的平均值（7.39 ± 0.24）高于广东（6.91 ± 0.28）。部分菌株检出5个非典型毒力基因。尤其是在60%以上的分离物中检测到flaCandvvh。海南的平均数量（2.30 +/- 0.20）明显高于广东（1.70 +/- 0.10）。多药耐药性普遍存在，对15种抗生素的平均耐药性为4.57 +/- 0.18。海南的抗生素耐药性和抗生素耐药基因的平均数量（分别为5.25 +/- 0.27和1.11 +/- 0.15）均高于广东（分别为3.87 +/- 0.21和0.75 +/- 0.10）。结果表明，海南省的病原菌毒力基因数量和耐药性均高于广东省，说明海南省的高温和抗生素污染可能会增强病原菌的耐药性和感染。
To understand the driving environmental factors in changes of bacterial virulence and antibiotic resistance, we determined the prevalence, antibiotic resistance and antibiotic resistance and virulence genes ofVibrio harveyiisolated from diseased marine fish in south coastal China. We isolated 2, 52 and 53V. harveyistrains from Fujian, Hainan and Guangdong, respectively, and identified them by multilocus sequence analysis of 16S rRNA-toxR(Vh)-rctB. Nine typical virulence genes were represented at a higher average in Hainan (7.39 +/- 0.24) than in Guangdong (6.91 +/- 0.28). Five atypical virulence genes were detected in some isolates. In particular,flaCandvvhwere detected in more than 60% of isolates. Their average number was significantly higher in Hainan (2.30 +/- 0.20) than in Guangdong (1.70 +/- 0.10). Multidrug resistance was widespread with an average resistance to 4.57 +/- 0.18 of 15 antibiotics. Both the average number of antibiotic resistance and antibiotic resistance genes were higher in Hainan (5.25 +/- 0.27 and 1.11 +/- 0.15, respectively) than in Guangdong (3.87 +/- 0.21 and 0.75 +/- 0.10, respectively). This study demonstrated that there were more virulence genes and greater drug resistance in Hainan than in Guangdong, suggesting that warmer temperature and antibiotics pollutants probably enhance antibiotic resistance and bacterial infection.