Distribution of the large aggregating proteoglycan versican in adult human tissues
Distribution of the large aggregating proteoglycan versican in adult human tissues
复制标题
DOI：
10.1177/44.4.8601689
复制
发表时间：
1996-04-01
影响因子：
3.2
通讯作者：
Zimmermann, DR
中科院分区：
文献类型：
--
作者：
BodeLesniewska, B;DoursZimmermann, MT;Zimmermann, DR
我们研究了分布的大透明质酸结合蛋白聚糖多功能蛋白聚糖（也称为PG-M）在成人组织中使用亲和纯化的多克隆抗体，识别的核心蛋白的突出的多功能蛋白聚糖剪接变体V0和V1。Versican存在于各种器官的疏松结缔组织中，并且通常与弹性纤维网络相关联。此外，它局限于大多数平滑肌组织和纤维和弹性软骨。在中枢和外周神经系统、表皮基底层和一些腺上皮的腔表面也观察到Versican染色。在血管中，versican存在于静脉和弹性动脉的所有三个壁层中。在肌性动脉中，免疫反应性通常局限于外膜的图尼卡。然而，它出现在动脉粥样硬化转化的血管壁的中膜和分裂的弹性内膜中。我们的调查，在正常人体组织中的多功能蛋白聚糖的分布，现在形成的基础上，潜在的异常多功能蛋白聚糖的表达在致病过程中的扩展研究。
We studied the distribution of the large hyaluronan-binding proteoglycan versican (also known as PG-M) in human adult tissues using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican was present in the loose connective tissues of various organs and was often associated with the elastic fiber network. Furthermore, it was localized in most smooth muscle tissues and in fibrous and elastic cartilage. Versican staining was also noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. In blood vessels, versican was present in all three wall layers of veins and elastic arteries. In muscular arteries the immunoreactivity was normally restricted to the tunica adventitia. However, it appeared in the media and the split elastica interna of atherosclerotically transformed vessel walls. Our survey of the distribution of versican in normal human tissues now forms the basis for extended studies of potentially aberrant versican expression during pathogenic processes.