PREPARATION AND SOLUTION PROPERTIES OF PULLULAN FRACTIONS AS STANDARD SAMPLES FOR WATER-SOLUBLE POLYMERS
PREPARATION AND SOLUTION PROPERTIES OF PULLULAN FRACTIONS AS STANDARD SAMPLES FOR WATER-SOLUBLE POLYMERS
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DOI：
10.1016/0144-8617(84)90049-3
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发表时间：
1984-01-01
影响因子：
11.2
通讯作者：
NAKAMURA, S
中科院分区：
文献类型：
--
作者：
KAWAHARA, K;OHTA, K;NAKAMURA, S
制备了一系列分子量在5 × 103 ~ 8 × 105之间的普鲁兰多糖组分。所有样品的重均分子量（Mw）通过沉降平衡测定。采用粘度法和超离心法研究了普鲁兰多糖在水溶液中的流体力学性质。实验结果表明，当分子量大于2 × 104时，普鲁兰多糖分子在水中是相当稳定的，表现为膨胀的无规线团。通过凝胶过滤测量级分的分子量分布。除了具有最高Mw的样品之外，Mw/Mn比接近1·1。可以得出结论，通过本工作制备的普鲁兰多糖级分具有良好的特征，并且具有窄的分子量分布。它们可用作研究水溶性聚合物的标准样品。
A series of pullulan fractions with molecular weights in the range 5 × 103to 8 × 105were prepared. The weight-average molecular weight (Mw) of all the samples was determined by sedimentation equilibrium. The hydrodynamic properties of pullulan in aqueous solution were investigated by viscometry and ultracentrifugation. The experimental results indicate that pullulan molecules in water are fairly stable and behave as expanded random coils when Mwis above 2 × 104. The molecular weight distributions of the fractions were measured by gel filtration. The ratio Mw/Mnwas close to 1·1, except for a sample with the highest Mw.It is concluded that the pullulan fractions prepared by the present work are well characterized and have a narrow molecular weight distribution. They may be useful as standard samples for studies of water-soluble polymers.