Toxicity to early life stages on medaka (Oryzias latipes) and in vitro estrogen intensity of bisphenol compounds
Toxicity to early life stages on medaka (Oryzias latipes) and in vitro estrogen intensity of bisphenol compounds
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DOI：
10.11403/jset.11.133
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
K. Yokota;C. Kato;M. Hirano;Hiroshi Ishibashib;H. Shiratsuchi;K. Tachibana;K. Arizono
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文献类型：
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作者：
K. Yokota;C. Kato;M. Hirano;Hiroshi Ishibashib;H. Shiratsuchi;K. Tachibana;K. Arizono
本研究以青鳉为材料，研究了双酚A（BPA）、双酚B（BPB）、双酚E（BPE）、双酚F（BPF）和双酚P（BPP）等6种双酚类化合物对青鳉早期生命阶段的毒性及其体外雌激素活性。BPs对受精后24 h以内胚胎的14 d LC_（50）值为2800 ~ 28600 μg/L（BPA：14800 μg/L，BPB：7400 μg/L，BPE：26000 μg/L，BPF：28600 μ g/L，BPP：2800 μ g/L）。BPs暴露14 d后孵化率的最低观测效应浓度分别为：BPA：12500 μg/L，BPB：8890 μg/L，BPE：26670 μ g/L，BPF：17780 μg/L，BPP：1780 μg/L。此外，在17780 μg/L的BPF剂量下，暴露于BPs 14天的受精卵的孵化时间显著延迟。BPs对24 h龄幼虫的96 h LC_（50）为6100 ~ 13900 μg/L（BPA：13900 μg/L，BPB：6100 μg/L，BPE：13900 μg/L，BPF：13300 μ g/L，BPP：2300 μg/L）。另一方面，BPA对青鳉雌激素受体α的雌激素活性估计为BPA：0。06，BPB：0。10，BPE：0。03，BPF：0。13，BPP：0。酵母双杂交检测雌二醇-17 β：100的表达量。这些结果表明，BPB和BPP对青鳉的早期生活阶段具有较高的毒性，BPB和BPF的体外雌激素活性高于BPA。
In this study, we investigated the toxicity of bisphenol compounds (BPs), namely, bisphenol A (BPA), bisphenol B (BPB), bisphenol E (BPE), bisphenol F (BPF) and bisphenol P (BPP) to the early life stages of medaka, and their estrogenic activity in vitro based on chemiluminescent signal intensity. The 14-d LC50 values of BPs on embryos less than 24-h post-fertilization were estimated in the range of 2800 to 28600 μg/L (BPA:14800 μg/L, BPB: 7400 μg/L, BPE: 26000 μg/L, BPF: 28600 μg/L and BPP: 2800 μ g/L). In addition, the lowest-observed-effect concentrations of hatchability after 14-d BPs exposure were estimated as follows: BPA: 12500 μg/L, BPB: 8890 μg/L, BPE: 26670 μ g/L, BPF: 17780 μg/L and BPP: 1780 μg/L. Moreover, the time to hatching of fertilized eggs exposed to BPs for 14 days was significantly delayed at a BPF dose of 17780 μg/L. The 96-h LC50 values of BPs on 24-h-old larvae were estimated in the range between 6100 and 13900 μg/L (BPA: 13900 μg/L, BPB: 6100 μg/L, BPE: 13900 μg/L, BPF: 13300 μg/L and BPP: 2300 μg/L). On the other hand, the estrogenic activities of BPs for medaka estrogen receptor α were estimated as BPA: 0. 06, BPB: 0. 10, BPE: 0. 03, BPF: 0. 13 and BPP: 0. 05 compared with estradiol-17β: 100 in the yeast two-hybrid assay. These results suggest that BPB and BPP have high toxicity to the early life stages of medaka and that the in vitro estrogenic activities of BPB and BPF were higher than that of BPA.