Antibacterial susceptibility patterns of Porphyromonas gingivalis isolated from chronic periodontitis patients
Antibacterial susceptibility patterns of Porphyromonas gingivalis isolated from chronic periodontitis patients
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DOI：
10.4317/medoral.17174
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发表时间：
2011-11-01
影响因子：
2.2
通讯作者：
Alborzi, Abdolvahab
中科院分区：
文献类型：
--
作者：
Japoni, Aziz;Vazin, Afsaneh;Alborzi, Abdolvahab
目的：为了测试牙龈卟啉单胞菌的抗菌药物的敏感性，一个小组的8个口服抗生素在慢性牙周病和评估因素与牙周炎在成人patients.Study Design：共50株牙龈卟啉单胞菌分离自120例成人慢性periodontitis。通过厌氧培养和生化试验对细菌进行鉴定。选择牙龈卟啉单胞菌菌落，评价青霉素、甲硝唑、阿莫西林、阿莫西林/克拉维酸、克林霉素、强力霉素、环丙沙星和阿奇霉素的抗菌活性。大多数患者经常刮治，感染牙牙周袋深度为5-8 mm，大多数患者在取样时出血。药敏试验显示，100%的牙龈卟啉单胞菌的阿奇霉素，强力霉素和阿莫西林/克拉维酸的敏感性，但较低的敏感性被发现为其余的抗生素agents.Conclusions：在40-50岁之间的妇女经常刮与慢性牙周炎呈正相关。结论慢性牙周炎患者牙龈卟啉单胞菌对抗生素的耐药水平较低，联合机械清创治疗时抗生素的应用可能反映了牙龈卟啉单胞菌的耐药水平。这可能表明，定期抗生素敏感性测试是必要的，以确定抗菌药物的疗效，如果慢性牙周病的机械清创后的完美治愈的意思。需要进一步的临床研究来证实体外结果。本研究的唯一限制是细菌鉴定为种属而非亚种水平。
Objectives: To test the antimicrobial sensitivity of Porphyromonas gingivalis to a panel of eight orally administrable antibiotics in chronic periodontal diseases and to evaluate factors associated with periodontitis in adult patients.Study Design: A total of fifty strains of P. gingivalis were isolated from one hundred and twenty adult patients with chronic perio-dontitis. Identification of bacteria was carried out by anaerobic culture and biochemical tests. Selected colonies of P. gingivalis were used to evaluate the antibacterial activities of penicillin, metronidazole, amoxicillin, amoxicillin/clavulanic acid, clindamycin, doxy-cycline, ciprofloxacin and azithromycin.Results: Most of the patients were female, age ranging between 40 to 50 years. Majority of the patients frequently had scaling and depths of periodontal pockets in infected teeth were 5-8 mm and most of them had hemorrhage during sampling. Susceptibility testing revealed a sensitivity of 100% of P. gingivalis to azithromycin, doxycycline and amoxicillin/clavulanic acid but lower susceptibilities were found for the rest of antibiotic agents evaluated.Conclusions: Frequent scaling in women aged between 40-50 years had positive correlation with chronic periodontitis. The application of antibiotics in conjuction with mechanical debridation, may reflect in the level of resistance of P. gingivalis in patients with chronic periodontal infections. This could suggest periodical antibiotic susceptibility testing is necessary to determine the efficacy of antimicrobial agents if the perfect curing of chronic periodontal diseases after mechanical debridation is meant. Further clinical studies are required to confirm the in vitro results. The only limitation in this study was identification of bacteria to species rather than subspecies level.