Separation of peptides by spiral countercurrent chromatography
Separation of peptides by spiral countercurrent chromatography
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DOI：
10.1080/10826070701812699
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发表时间：
2008-01-01
影响因子：
1.3
通讯作者：
Finn, Thomas M.
中科院分区：
文献类型：
--
作者：
Knight, Martha;Ito, Yoichiro;Finn, Thomas M.
螺旋逆流色谱采用了一种新型的分离转子，该分离转子由8块带有螺旋流道的高密度聚乙烯板组成，安装在J型盘管行星离心机上。对合成肽在不同溶剂体系中的分配系数进行了评价。对大约30毫克的量进行了层析，并分析了这些级分的多肽含量和纯度。所选择的含仲丁醇或正丁醇的溶剂体系和流动相具有较好的纯化效果。固定相的保留率高达60%以上，因此螺旋分离线圈能够保留这些粘度较高、界面张力较低的溶剂，这些溶剂已不能很好地用于高速CCC的多层线圈。
Spiral countercurrent chromatography (CCC) utilizes a new separation rotor composed of 8 high density polyethylene plates with spiral flow channels, mounted in the type-J coil planet centrifuge (CPC). Synthetic peptides were evaluated for partition coefficients in various solvent systems. Approximately 30mg amounts were chromatographed and the fractions analyzed for peptide content and purity. The solvent systems which contained sec-butanol or n-butanol and the mobile phase selected produced good purification. The stationary phase was highly retained over 60%, thus the spiral separation coil is able to retain these more viscous, low interfacial tension solvents that have not been able to be used well in the multi-layer coil for high speed CCC.