Production of Succinate from Acetate by Metabolically Engineered Escherichia coli
Production of Succinate from Acetate by Metabolically Engineered Escherichia coli
复制标题
通过代谢工程大肠杆菌从乙酸盐生产琥珀酸
DOI：
10.1021/acssynbio.6b00052
复制
发表时间：
2016-11-01
影响因子：
4.7
通讯作者：
Zhang, Y-H Percival
中科院分区：
文献类型：
--
作者：
Li, Yunjie;Huang, Bing;Zhang, Y-H Percival
乙酸盐是工业生物废水和木质纤维素生物质水解产物的主要成分，可能是一种成本较低的替代碳源。在这里，我们设计了大肠杆菌 MG1655 菌株，用于以乙酸盐作为唯一碳源生产琥珀酸。代谢工程策略包括阻断 TCA 循环、重定向糖异生途径以及增强乙醛酸分流。工程菌株 MG03 缺失了以下基因：琥珀酸脱氢酶 (sdhAB)、异柠檬酸裂解酶调节剂 (iclR) 和苹果酸酶 (maeB)，在 72 小时内积累了 6.86 mM 的琥珀酸。过表达柠檬酸合酶(g1tA)的MG03(pTrc99a-gltA)积累了16.45 mM的琥珀酸，产量达到0.46 mol/mol，约为最大理论产量的92%。采用静息池将乙酸转化为琥珀酸，琥珀酸的最高浓度达到61.7 mM。
Acetate, a major component of industrial biological wastewater and of lignocellulosic biomass hydrolysate, could potentially be a less costly alternative carbon source. Here we engineered Escherichia coli MG1655 strain for succinate production from acetate as the sole carbon source. Strategies of metabolic engineering included the blockage of the TCA cycle, redirection of the gluconeogenesis pathway, and enhancement of the glyoxylate shunt. The engineered strain MG03 featuring the deletion of genes: succinate dehydrogenase (sdhAB), isocitrate lyase regulator (iclR), and malic enzymes (maeB) accumulated 6.86 mM of succinate in 72 h. MG03(pTrc99a-gltA) overexpressing citrate synthase (g1tA) accumulated 16.45 mM of succinate and the yield reached 0.46 mol/mol, about 92% of the maximum theoretical yield. Resting-cell was adopted for the conversion of acetate to succinate, and the highest concentration of succinate achieved 61.7 mM.