Homeobox genes in the cnidarian Eleutheria dichotoma: evolutionary implications for the origin of Antennapedia-class (HOM/Hox) genes.
Homeobox genes in the cnidarian Eleutheria dichotoma: evolutionary implications for the origin of Antennapedia-class (HOM/Hox) genes.
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刺胞动物 Eleutheria dichotoma 中的同源盒基因：触角类 (HOM/Hox) 基因起源的进化意义。
DOI：
10.1006/mpev.1996.0055
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发表时间：
1996
影响因子：
4.1
通讯作者：
Bernd Schierwater
中科院分区：
文献类型：
--
作者：
K. Kuhn;Bruno Streit;Bernd Schierwater;Bernd Schierwater
为了验证目前关于后生动物中Antp-class （HOM/Hox）基因早期进化的假设，我们调查了双胚层水螅Eleutheria dichotoma中这些基因的存在。利用不同组的简并引物进行PCR，鉴定出3个新的同源盒序列，以及之前发现的2个片段。在3‘和5’侧区进行RACE扩增后，我们将cox -1至cox -5这5个基因归类为antp类。同源结构域与果蝇和人类最相似的HOM/Hox基因的同源性在58%至72%之间。每一个Cnox基因都含有不止一个从三胚层动物中已知的antp类家族的诊断残基。这些数据与先前提出的祖先HOM/Hox集群组成不一致，涉及发现的antp类同源盒基因的数量和类型。
In order to test current hypotheses on the early evolution of Antp-class (HOM/Hox) genes in metazoan animals, we surveyed the diploblastic hydrozoan Eleutheria dichotoma for the presence of these genes. By means of PCR with different sets of degenerate primers, three new homeobox sequences were identified, in addition to two previously found fragments. Following RACE amplification of the 3' and 5' flanking regions, we classified all five genes, Cnox-1 to Cnox-5, as belonging to the Antp-class. Homeodomain identity to the most similar HOM/Hox genes from Drosophila and human ranges from 58 to 72%. Each of the Cnox genes contains diagnostic residues of more than one Antp-class family known from triploblastic animals. These data are at odds with a previously proposed ancestral HOM/Hox cluster composition, with respect to both the number and types of Antp-class homeobox genes found.