Alginase enzyme production by Bacillus circulans
Alginase enzyme production by Bacillus circulans
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环状芽孢杆菌生产海藻酶
DOI：
--
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发表时间：
1984
影响因子：
4.4
通讯作者：
J. Ram
中科院分区：
文献类型：
--
作者：
J. Hansen;R. Doubet;J. Ram
对溪流和土壤样品进行了筛选，以确定是否有微生物可以利用来自粘液铜绿假单胞菌的藻酸盐作为唯一的碳源和能源。分离含有大的需氧杆菌的纯培养物。细胞大小约为0.8 × 2.5微米，具有侧生或周生鞭毛，革兰氏染色反应阴性，并在孢子形成培养基上产生孢子。通过热变性测量，纯化的DNA为约46mol%G +C。根据这些和其他生化测试，该生物被鉴定为环状芽孢杆菌。培养基中出现了降解褐藻胶的酶活性。凝胶过滤后，藻酸酶活性在对应于40，000道尔顿蛋白质的位置处洗脱为单峰。从该一步部分纯化回收的活性显示出明显的内切甘露糖醛酸酶特异性。与以前报道的其他藻酸酶一样，该酶似乎可能是裂解酶（或消除酶）。然而，迄今为止还没有报道芽孢杆菌属物种或其它革兰氏阳性细菌产生具有藻酸酶活性的胞外酶。其他几个B。来自美国典型培养物保藏中心的circulans菌株似乎也具有可诱导的胞外藻酸酶活性。
Stream and soil samples were screened for microorganisms that would use alginate from mucoid Pseudomonas aeruginosa as the sole carbon and energy source. A pure culture containing large aerobic rods was isolated. The cells were about 0.8 by 2.5 microns in size, had lateral or peritrichous flagella, had a negative Gram stain reaction, and produced spores on sporulation medium. Purified DNA was approximately 46 mol% G+C as measured by thermal denaturation. From these and other biochemical tests, the organism was identified as Bacillus circulans. The enzyme activity that degraded alginate appeared in the culture medium. Upon gel filtration, alginase activity eluted as a single peak at a position corresponding to a protein of 40,000 daltons. Activity recovered from this one-step, partial purification showed apparent endomannuronidase specificity. Like other alginases previously reported, the enzyme appeared likely to be a lyase (or eliminase). However, no Bacillus species or other gram-positive bacteria have heretofore been reported to produce extracellular enzymes with alginase activity. Several other B. circulans strains from the American Type Culture Collection also appeared to have inducible extracellular alginase activity.
囊性纤维化中的粘液大肠杆菌。
DOI：
10.1056/nejm198106113042401
发表时间：
1981
期刊：
The New England journal of medicine
影响因子：
--
作者：
Macone,AB;Pier,GB;Pennington,JE;MatthewsJr,WJ;Goldmann,DA
通讯作者：
Goldmann,DA