Increased level of arginase activity correlates with disease severity in HIV-seropositive patients.
Increased level of arginase activity correlates with disease severity in HIV-seropositive patients.
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DOI：
10.1086/653736
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发表时间：
2010-08-15
期刊：
影响因子：
--
通讯作者：
Kropf P
中科院分区：
文献类型：
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作者：
Cloke TE;Garvey L;Choi BS;Abebe T;Hailu A;Hancock M;Kadolsky U;Bangham CR;Munder M;Müller I;Taylor GP;Kropf P
感染人类免疫缺陷病毒（HIV）导致慢性感染，逐渐损害免疫系统。虽然CD4+ T细胞的耗竭经常被用来解释免疫抑制，但慢性感染和CD4+ T细胞的进行性损失不足以完全解释免疫失调。精氨酸酶诱导的l-精氨酸剥夺正在成为免疫应答下调的关键机制。在这里，我们假设精氨酸酶活性水平随着hiv血清阳性患者疾病严重程度的增加而增加。我们测定了hiv血清阳性患者和未感染对照参与者外周血单个核细胞精氨酸酶活性水平。我们的研究结果表明，与CD4+ T细胞计数高的患者或未感染的对照组相比，CD4+ T细胞计数低的hiv血清阳性患者的外周血单个核细胞表达的精氨酸酶活性水平具有统计学意义上的显著性升高。此外，我们发现在hiv血清阳性患者中，高精氨酸酶活性水平与高病毒载量之间存在统计学上显著的相关性。
Infection with human immunodeficiency virus (HIV) results in a chronic infection that progressively impairs the immune system. Although depletion of CD4+ T cells is frequently used to explain immunosuppression, chronicity of infection and progressive loss of CD4+ T cells are not sufficient to fully account for immune dysregulation. Arginase-induced l-arginine deprivation is emerging as a key mechanism for the down-regulation of immune responses. Here, we hypothesized that arginase activity levels increase with disease severity in HIV-seropositive patients. We determined the levels of arginase activity in peripheral blood mononuclear cells from HIV-seropositive patients and uninfected control participants. Our results show that peripheral blood mononuclear cells from HIV-seropositive patients with low CD4+ T cell counts expressed statistically significantly higher levels of arginase activity, compared with patients with high CD4+ T cell counts or with uninfected control participants. Furthermore, we found a statistically significant correlation between high arginase activity level and high viral load in HIV-seropositive patients.