Perineuronal nets affect parvalbumin expression in GABAergic neurons of the mouse hippocampus.
Perineuronal nets affect parvalbumin expression in GABAergic neurons of the mouse hippocampus.
复制标题
神经周围网影响小鼠海马 GABA 能神经元中小白蛋白的表达。
DOI：
10.1111/ejn.12792
复制
发表时间：
2014
期刊：
影响因子：
3.4
通讯作者：
Jinno S.
中科院分区：
文献类型：
--
作者：
Yamada J;Ohgomori T;Jinno S.
最近的研究表明，神经元周网（PNN），一种专门的细胞外基质结构，和小清蛋白（PV），一种EF手钙结合蛋白，参与调节神经回路的可塑性。在这里，我们的目的是定量估计两个可塑性调节剂，PV和PNNs，在年轻的成年小鼠海马之间的关系。PV和紫藤凝集素（一种广泛的PNN标记物）的双重荧光染色显示，大量PV表达（PV+）GABA能神经元缺乏PNN。光学显微镜分析显示PNN+神经元数量少于PV+神经元。PV+神经元中PNN表达的比率在树突层中一般低于在主细胞层中，而PV+神经元中PNN表达的比率有效地为100%。PNN+/PV+神经元的平均PV荧光显著高于PNN−/PV+神经元。单细胞PV荧光的累积频率表明，强染色的PV+神经元倾向于被PNN覆盖，而弱染色的PV+神经元可能缺乏PNN。我们通过单侧注射软骨素酶ABC（chABC）到背侧CA 1区消化PNNs。虽然PV+神经元的密度保持不变，但PV荧光在注射chABC后7天下降。定量真实的实时聚合酶链反应分析表明，注射chABC后PV mRNA表达降低。这些发现表明，PNNs的存在或不存在影响海马GABA能神经元中PV的相对表达。
Recent studies have suggested that the perineuronal net (PNN), a specialised extracellular matrix structure, and parvalbumin (PV), an EF‐hand calcium‐binding protein, are involved in the regulation of plasticity of neural circuits. Here, we aimed to quantitatively estimate the relationship between the two plasticity regulators, PV and PNNs, in the hippocampus of young adult mice. Dual fluorescence staining for PV andWisteria floribundaagglutinin (a broad PNN marker) showed that a substantial population of PV‐expressing (PV+) GABAergic neurons lacked PNNs. Optical disector analysis demonstrated that there were fewer PNN+neurons than PV+neurons. The ratio of PNN expression in PV+neurons was generally lower in the dendritic layers than in the principal cell layers, whereas the ratio of PV expression in PNN+neurons was effectively 100%. The mean PV fluorescence was significantly higher in PNN+/PV+neurons than in PNN−/PV+neurons. Cumulative frequencies for single‐cell PV fluorescence indicated that intensely stained PV+neurons tend to be enwrapped by PNNs, whereas weakly stained PV+neurons are likely to lack PNNs. We digested the PNNs by a unilateral injection of chondroitinase ABC (chABC) into the dorsal CA1 region. Although the densities of PV+neurons remained unchanged, the PV fluorescence declined 7 days after chABC injection. Quantitative real‐time polymerase chain reaction analysis demonstrated a reduction in PV mRNA expression following chABC injection. These findings indicate that the presence or absence of PNNs affects the relative PV expression in GABAergic neurons in the hippocampus.