Detection and analysis of six lizard adenoviruses by consensus primer PCR provides further evidence of a reptilian origin for the atadenoviruses
Detection and analysis of six lizard adenoviruses by consensus primer PCR provides further evidence of a reptilian origin for the atadenoviruses
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DOI：
10.1128/jvi.78.23.13366-13369.2004
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发表时间：
2004-12-01
影响因子：
5.4
通讯作者：
Jacobson, ER
中科院分区：
文献类型：
--
作者：
Wellehan, JFX;Johnson, AJ;Jacobson, ER
设计了一种用于腺病毒DNA聚合酶基因研究的一致性巢式PCR方法。从7种蜥蜴中的6种新型腺病毒中扩增基因片段并进行测序，其中包括4种以前从未报道过的腺病毒。宿主物种包括毒蜥、豹壁虎、肥尾壁虎、蓝舌石龙子、东海壁虎、须蜥和山地变色龙。这是蜥蜴腺病毒的第一个序列信息。系统发育分析表明，这些病毒属于Atadenovirus属，支持atadenovirus的爬行动物起源。该PCR方法可用于获得用于新型腺病毒的初始测序的模板。
A consensus nested-PCR method was designed for investigation of the DNA polymerase gene of adenoviruses. Gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. Host species included Gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, Tokay gecko, bearded dragon, and mountain chameleon. This is the first sequence information from lizard adenoviruses. Phylogenetic analysis indicated that these viruses belong to the genus Atadenovirus, supporting the reptilian origin of atadenoviruses. This PCR method may be useful for obtaining templates for initial sequencing of novel adenoviruses.