Early detection of cerebral ischemic damage and repair process in the gerbil by use of an immunohistochemical technique.
Early detection of cerebral ischemic damage and repair process in the gerbil by use of an immunohistochemical technique.
复制标题
利用免疫组织化学技术早期检测沙鼠脑缺血损伤和修复过程。
DOI：
10.1016/s0025-6196(12)65471-8
复制
发表时间：
1987
影响因子：
8.9
通讯作者：
Yanagihara,T
中科院分区：
文献类型：
--
作者：
Matsumoto,M;Yamamoto,K;Homburger,HA;Yanagihara,T
结扎沙土鼠右颈总动脉后，以微管蛋白、肌酸激酶BB同工酶(CK-BB)、神经元特异性烯醇化酶为神经元标记物，以星形胶质细胞标记物星形胶质细胞蛋白、胶质纤维酸性蛋白、CK-BB为星形细胞标记物，对沙土鼠脑缺血损伤和脑缺血后损伤的进展及修复过程进行免疫组织化学检测。缺血5min后，最早的缺血灶出现在海马区和大脑皮层，表现为神经纤维、神经细胞体和树突的反应消失。树突中的反应比神经细胞体中的反应更迅速地消失。微管蛋白反应是检测神经元缺血性损伤的最敏感指标。在30分钟的缺血期和随后的脑循环重建之后，影响神经元结构的免疫组织化学损伤在最初的3小时内扩大，然后缓慢地随后最多12小时。再灌流24小时后即可检测到反应性星形胶质细胞。目前的研究表明，在脑缺血发生后不久，免疫组织化学技术可以清楚地显示早期的缺血性损伤，但不仅在不同的解剖区域，而且在神经元结构内也存在着相当大的异质性。这项技术与其他形态学、生理学或生化技术结合使用时，有可能进一步研究脑缺血或其他病理生理状况。
After occlusion of the right common carotid artery in the gerbil, we monitored the progression of ischemic damage and postischemic damage and the repair process in the brain immunohistochemically by using tubulin, creatine kinase BB-isoenzyme (CK-BB), and neuron-specific enolase as the neuronal markers and astroprotein, glial fibrillary acidic protein, and CK-BB as the astrocytic markers. The earliest ischemic lesion was detected in the hippocampus and the cerebral cortex after ischemia for 5 minutes as loss of the reaction in the neuropil, nerve cell bodies, and dendrites. The reaction disappeared more promptly in the dendrites than in the nerve cell bodies. The reaction for tubulin was the most sensitive for detection of the neuronal ischemic damage. After an ischemic period of 30 minutes and subsequent reestablishment of cerebral circulation, the immunohistochemical lesions affecting the neuronal structure expanded during the first 3 hours and then slowly afterward for up to 12 hours. Reactive astrocytes were already identified 24 hours after reperfusion. The current investigation demonstrated that early ischemic damage can be clearly visualized by use of the immunohistochemical technique soon after the onset of cerebral ischemia but that considerable heterogeneity exists not only in different anatomic regions but also within the neuronal structure. This technique has potential for further investigation of cerebral ischemia or other pathophysiologic conditions when used in combination with other morphologic, physiologic, or biochemical techniques.