Exogenous ATP and other nucleoside phosphates modulate epidermal growth factor receptors of A-431 epidermoid carcinoma cells.
Exogenous ATP and other nucleoside phosphates modulate epidermal growth factor receptors of A-431 epidermoid carcinoma cells.
复制标题
外源 ATP 和其他核苷磷酸调节 A-431 表皮样癌细胞的表皮生长因子受体。
DOI：
10.1073/pnas.86.12.4510
复制
发表时间：
1989
影响因子：
11.1
通讯作者：
Edidin,M
中科院分区：
文献类型：
--
作者：
Hosoi,K;Edidin,M
A-431人表皮样癌细胞在37℃暴露于低浓度(0.01-1 mM)的三磷酸腺苷和其他核苷5‘-三磷酸后，对表皮生长因子(EGF)的结合力降低，但在0℃时不受影响。这是由于失去高亲和力的EGF结合部位所致。这种调节与肌醇磷酸合成和细胞内钙离子的瞬时增加以及丝氨酸和苏氨酸上EGF受体的磷酸化有关。没有证据表明标记的ATP进入了细胞。ATP似乎能与特定的细胞表面受体结合。这种结合直接与非代谢的三磷酸腺苷类似物5‘-[β，伽马亚胺]三磷酸结合。
The binding of epidermal growth factor (EGF) by A-431 human epidermoid carcinoma cells was reduced after exposure of the cells to low concentrations (0.01-1 mM) of ATP and other nucleoside 5'-triphosphates at 37 degrees C, but not at 0 degree C. This was due to loss of high-affinity EGF binding sites. The modulation was associated with transient increases in inositol phosphate synthesis and intracellular Ca2+ and with phosphorylation of the EGF receptor on serine and threonine. There was no evidence for entry of labeled ATP into the cells. ATP appeared to bind to specific cell surface receptors. Such binding was demonstrated directly with the nonmetabolizable ATP analogue adenosine 5'-[beta,gamma-imido]triphosphate.