Plasma-Membrane-Localized Transporter NREET1 is Responsible for Rare Earth Element Uptake in Hyperaccumulator Dicranopteris linearis.
Plasma-Membrane-Localized Transporter NREET1 is Responsible for Rare Earth Element Uptake in Hyperaccumulator Dicranopteris linearis.
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DOI：
10.1021/acs.est.2c09320
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发表时间：
2023-04
影响因子：
11.4
通讯作者：
Hong-Xiang Zheng;Wenshen Liu;Dan Sun;Shishu Zhu;Yang Li;Yu-Lu Yang;Ruo-Rong Liu;Hua–Yuan Feng-Hua–Yu
中科院分区：
文献类型：
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作者：
Hong-Xiang Zheng;Wenshen Liu;Dan Sun;Shishu Zhu;Yang Li;Yu-Lu Yang;Ruo-Rong Liu;Hua–Yuan Feng-Hua–Yu
稀土元素（ree）对许多现代技术至关重要，全球需求不断增加；然而，生产步骤是资源密集型的，对环境有害。一些植物物种能够过度积累稀土元素，了解这一现象背后的生物学原理可以在开发更环保的稀土元素回收技术中发挥关键作用。在这里，我们从稀土超富集植物蕨类Dicranopteris linearis中鉴定了一个稀土转运蛋白NRAMP REE transporter 1 （NREET1）。虽然NREET1属于天然耐药相关巨噬细胞蛋白（natural resistance-associated macrophage protein， NRAMP）家族，但它与其他NRAMP成员的相似性较低。当在酵母中表达时，NREET1表现出稀土元素运输能力，但它不能运输二价金属，如锌、镍、锰或铁。NREET1主要表达于根中，主要定位于质膜中。在拟南芥中的表达研究表明，NREET1作为一种转运体，介导稀土元素从根细胞壁吸收并转移到细胞质中。此外，NREET1对轻稀土的亲和性高于重稀土，这与大田生长的线状木对轻稀土的优先富集是一致的。因此，我们得出结论，NREET1可能在D. lineis的摄取和随后的稀土超积累中起重要作用。这些发现为利用合成生物学技术设计和生产可持续的、基于植物的稀土元素回收系统奠定了基础。
Rare earth elements (REEs) are critical for numerous modern technologies, and demand is increasing globally; however, production steps are resource-intensive and environmentally damaging. Some plant species are able to hyperaccumulate REEs, and understanding the biology behind this phenomenon could play a pivotal role in developing more environmentally friendly REE recovery technologies. Here, we identified a REE transporter NRAMP REE Transporter 1 (NREET1) from the REE hyperaccumulator fern Dicranopteris linearis. Although NREET1 belongs to the natural resistance-associated macrophage protein (NRAMP) family, it shares a low similarity with other NRAMP members. When expressed in yeast, NREET1 exhibited REE transport capacity, but it could not transport divalent metals, such as zinc, nickel, manganese, or iron. NREET1 is mainly expressed in D. linearis roots and predominantly localized in the plasma membrane. Expression studies in Arabidopsis thaliana revealed that NREET1 functions as a transporter mediating REE uptake and transfer from root cell walls into the cytoplasm. Moreover, NREET1 has a higher affinity for transporting light REEs compared to heavy REEs, which is consistent to the preferential enrichment of light REEs in field-grown D. linearis. We therefore conclude that NREET1 may play an important role in the uptake and consequently hyperaccumulation of REEs in D. linearis. These findings lay the foundation for the use of synthetic biology techniques to design and produce sustainable, plant-based REE recovery systems.