Transcription of rDNA is essential for satellite association.
Transcription of rDNA is essential for satellite association.
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rDNA 的转录对于卫星关联至关重要。
DOI：
10.1159/000133666
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Henderson,AS
中科院分区：
文献类型：
--
作者：
McDowell,KA;Hadjiargyrou,M;Patsalis,P;Henderson,AS
分析了含有转染的人核糖体DNA的非近端着丝粒染色体与近端着丝粒染色体的非随机卫星关联。测试了两个结构。第一个包含完整的rDNA转录单位加上侧翼DNA，并在转染后插入染色体3p13→p12。该区域为银阳性，表明转录活跃。转录活性的rDNA参与卫星协会与其他rDNA轴承染色体的比率15%以上的随机背景协会。第二个构建体缺乏启动子区。该rDNA整合于染色体4q31→q32，银染阴性。未观察到与其他携带rDNA的染色体的非随机关联。这项研究表明，非随机卫星协会的关键DNA是rDNA本身，主动转录是一个必要条件。
A nonacrocentric human chromosome containing transfected human ribosomal DNA was analyzed for participation in nonrandom satellite associations with acrocentric chromosomes. Two constructs were tested. The first contained the complete rDNA transcriptional unit plus flanking DNA and was inserted at chromosome 3p13→p12 following transfection. The region was silver-positive indicating active transcription. The transcriptionally active rDNA participated in satellite association with other rDNA-bearing chromosomes at a rate 15 % above random background associations. The second construct lacked the promoter region. This rDNA integrated at chromosome 4q31→q32, and was silver stain-negative. Non-random associations with other rDNA-bearing chromosomes were not observed. The study demonstrates that the DNA critical to nonrandom satellite associations is rDNAper seand that active transcription is a requirement.