Replacing fishmeal with plant protein in Atlantic salmon (Salmo salar) diets by supplementation with fish protein hydrolysate
Replacing fishmeal with plant protein in Atlantic salmon (Salmo salar) diets by supplementation with fish protein hydrolysate
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DOI：
10.1038/s41598-020-60325-7
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发表时间：
2020-03-06
影响因子：
4.6
通讯作者：
Stanton, C.
中科院分区：
文献类型：
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作者：
Egerton, S.;Wan, A.;Stanton, C.
研究了添加和不添加鱼蛋白水解物（FPH）的80%植物蛋白饲料对大西洋鲑鱼生长和肠道健康的影响。给鱼喂食（A）含有35%鱼粉的对照饮食，（B）含有15%鱼粉的80%植物蛋白饮食，（C）含有5%鱼粉和10%部分水解蛋白的80%植物蛋白饮食，或（D）含有5%鱼粉和10%可溶性水解蛋白的80%植物蛋白饮食。80%植物-15%鱼粉饮食中的鱼明显小于其他饮食组中的鱼。然而，部分水解的蛋白质补充剂允许鱼生长，以及鱼喂养的控制35%的鱼粉饮食。喂食FPH饮食（饮食C和D）的鱼在其血液中具有显著更高的氨基酸水平，包括分别比35%鱼粉饮食的鱼多48%和27%的支链氨基酸。植物蛋白显著改变了肠道微生物组成，显著降低了α多样性。螺旋体和家庭Moritellaceae，Psychromonadaceae，Helicobacteraceae和Bacteroidaceae都被发现在显着较低的丰度组相比，80%的植物蛋白饲料的控制鱼粉饮食。
The effects of feeding an 80% plant protein diet, with and without fish protein hydrolysate (FPH) supplementation, on the growth and gut health of Atlantic salmon were investigated. Fish were fed either (A) a control diet containing 35% fishmeal, (B) an 80% plant protein diet with 15% fishmeal, (C) an 80% plant protein diet with 5% fishmeal and 10% partly hydrolysed protein, or (D) an 80% plant protein diet with 5% fishmeal and 10% soluble protein hydrolysate. Fish on the 80% plant- 15% fishmeal diet were significantly smaller than fish in the other dietary groups. However, partly-hydrolysed protein supplementation allowed fish to grow as well as fish fed the control 35% fishmeal diet. Fish fed the FPH diets (diets C and D) had significantly higher levels of amino acids in their blood, including 48% and 27% more branched chain amino acids compared to fish on the 35% fishmeal diet, respectively. Plant protein significantly altered gut microbial composition, significantly decreasing alpha -diversity. Spirochaetes and the families Moritellaceae, Psychromonadaceae, Helicobacteraceae and Bacteroidaceae were all found at significantly lower abundances in the groups fed 80% plant protein diets compared to the control fishmeal diet.