Smooth muscle protein 22 alpha-Cre is expressed in myeloid cells in mice.
Smooth muscle protein 22 alpha-Cre is expressed in myeloid cells in mice.
复制标题
DOI：
10.1016/j.bbrc.2012.05.041
复制
发表时间：
2012-06-15
影响因子：
3.1
通讯作者：
Mortensen RM
中科院分区：
文献类型：
--
作者：
Shen Z;Li C;Frieler RA;Gerasimova AS;Lee SJ;Wu J;Wang MM;Lumeng CN;Brosius FC 3rd;Duan SZ;Mortensen RM
利用Cre重组酶研究平滑肌特异性功能的实验依赖于Cre转基因表达的严格特异性。因此，准确测定Cre活性对于解释使用平滑肌特异性Cre的实验至关重要。将两系平滑肌蛋白22a-Cre （SM22a-Cre）小鼠与floxed小鼠杂交，以确定Cre的转基因表达。Southern blotting表明SM22a-Cre不仅在平滑肌丰富的组织中表达，也在脾脏中表达，脾脏主要由骨髓和淋巴样细胞等免疫细胞组成。PCR检测外周血和腹膜巨噬细胞中SM22a-Cre的表达。SM22a-Cre小鼠与重组检测器GFP小鼠杂交，流式细胞术显示循环中性粒细胞和单核细胞中表达GFP并因此重组。SM22 -Cre不仅介导平滑肌细胞的重组，也介导骨髓细胞（包括中性粒细胞、单核细胞和巨噬细胞）的重组。鉴于骨髓细胞对心血管表型的已知贡献，在使用SM22 -Cre小鼠解释数据以研究平滑肌特异性功能时应谨慎。当SM22 -Cre用于区分平滑肌细胞与骨髓细胞对观察到的表型的贡献时，骨髓移植等策略可能是必要的。
Experiments using Cre recombinase to study smooth muscle specific functions rely on strict specificity of Cre transgene expression. Therefore, accurate determination of Cre activity is critical to the interpretation of experiments using smooth muscle specific Cre. Two lines of smooth muscle protein 22 a-Cre (SM22a-Cre) mice were bred to floxed mice in order to define Cre transgene expression. Southern blotting demonstrated that SM22a-Cre was expressed not only in tissues abundant of smooth muscle, but also in spleen, which consists largely of immune cells including myeloid and lymphoid cells. PCR detected SM22a-Cre expression in peripheral blood and peritoneal macrophages. Analysis of SM22a-Cre mice crossed with a recombination detector GFP mouse revealed GFP expression, and hence recombination, in circulating neutrophils and monocytes by flow cytometry. SM22 -Cre mediates recombination not only in smooth muscle cells, but also in myeloid cells including neutrophils, monocytes, and macrophages. Given the known contributions of myeloid cells to cardiovascular phenotypes, caution should be taken when interpreting data using SM22 -Cre mice to investigate smooth muscle-specific functions. Strategies such as bone marrow transplantation may be necessary when SM22 -Cre is used to differentiate the contribution of smooth muscle cells versus myeloid cells to observed phenotypes.
登录
查看更多内容
影响因子：
8.3
作者：
Liao, Tang-Dong;Yang, Xiao-Ping;Carretero, Oscar A.
通讯作者：
Carretero, Oscar A.
影响因子：
11.2
作者：
Proweller, Aaron;Lili Tu;Parmacek, Mchael S.
通讯作者：
Parmacek, Mchael S.
影响因子：
20.1
作者：
Duan, SZ;Ivashchenko, CY;Mortensen, RM
通讯作者：
Mortensen, RM
影响因子：
4.6
作者：
Xin, HB;Deng, KY;Kotlikoff, MI
通讯作者：
Kotlikoff, MI
影响因子：
5.3
作者：
Akiyama, TE;Sakai, S;Gonzalez, FJ
通讯作者：
Gonzalez, FJ