Nitrogen stress response and stringent response are coupled in Escherichia coli.
Nitrogen stress response and stringent response are coupled in Escherichia coli.
复制标题
DOI：
10.1038/ncomms5115
复制
发表时间：
2014-06-20
影响因子：
16.6
通讯作者：
Wigneshweraraj, Sivaramesh
中科院分区：
文献类型：
--
作者：
Brown, Daniel R.;Barton, Geraint;Pan, Zhensheng;Buck, Martin;Wigneshweraraj, Sivaramesh
氮的同化是细菌必不可少的过程。氮调控应激反应是缺氮大肠杆菌清除替代氮源的适应性机制，需要全局转录调控因子NtrC。此外，缺氮的大肠杆菌细胞还会合成一种信号分子——四磷酸鸟苷（ppGpp），该信号分子作为包括转录在内的许多过程的效应分子，启动全球生理变化，统称为严格反应。在营养胁迫下导致ppGpp水平升高的调控机制仍然是未知的。在这里，我们发现NtrC在N饥饿期间激活了relA的转录，relA是负责ppGpp合成的关键基因。结果表明，NtrC结合了这两种主要的细菌应激反应来管理氮限制条件，并为特定营养应激如何导致细菌中ppGpp水平升高提供了新的机制见解。
Assimilation of nitrogen is an essential process in bacteria. The nitrogen regulation stress response is an adaptive mechanism used by nitrogen-starved Escherichia coli to scavenge for alternative nitrogen sources and requires the global transcriptional regulator NtrC. In addition, nitrogen-starved E. coli cells synthesize a signal molecule, guanosine tetraphosphate (ppGpp), which serves as an effector molecule of many processes including transcription to initiate global physiological changes, collectively termed the stringent response. The regulatory mechanisms leading to elevated ppGpp levels during nutritional stresses remain elusive. Here, we show that transcription of relA, a key gene responsible for the synthesis of ppGpp is activated by NtrC during N starvation. The results reveal that NtrC couples these two major bacterial stress responses to manage conditions of nitrogen limitation, and provide novel mechanistic insights into how a specific nutritional stress leads to elevating ppGpp levels in bacteria.
登录
查看更多内容
影响因子：
3.7
作者：
Atkinson GC;Tenson T;Hauryliuk V
通讯作者：
Hauryliuk V
DOI：
10.1073/pnas.1001188107
发表时间：
2010-05-18
影响因子：
11.1
作者：
Burrows, Patricia C.;Joly, Nicolas;Buck, Martin
通讯作者：
Buck, Martin
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.1073/pnas.88.5.1631
发表时间：
1991-03-01
影响因子：
11.1
作者：
CLAVERIEMARTIN, F;MAGASANIK, B
通讯作者：
MAGASANIK, B
影响因子：
3.2
作者：
Jiang, P;Ninfa, AJ
通讯作者：
Ninfa, AJ