Behaviour of liposomes loaded with bovine serum albumin during in vitro digestion
Behaviour of liposomes loaded with bovine serum albumin during in vitro digestion
复制标题
牛血清白蛋白脂质体体外消化过程中的行为
DOI：
10.1016/j.foodchem.2014.11.108
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发表时间：
2015-05-15
期刊：
影响因子：
8.8
通讯作者：
Singh, Harjinder
中科院分区：
文献类型：
--
作者：
Liu, Weilin;Ye, Aigian;Singh, Harjinder
本研究考察了负电荷蛋白质(牛血清白蛋白，BSA)脂质体在体外消化过程中的稳定性。Zeta-电位和形态测量证实成功制备了牛血清白蛋白脂质体，包封率约为34%。在模拟胃环境中消化时，包被的BSA和脂质体的完整性不随时间变化，这表明脂膜保护了包埋的BSA不受胃蛋白酶的影响。载牛血清白蛋白脂质体在模拟肠液中的稳定性较差，表现为细胞膜受损和游离脂肪酸的释放。此外，脂解动力学表明，胆盐和离子强度可以促进高水平的游离脂肪酸释放。这项工作进一步加深了我们对胃肠道消化条件对脂质体性质影响的认识，并为食品和保健行业的脂质体制剂的设计提供了有价值的信息。(C)2014爱思唯尔有限公司。保留所有权利。
This study examined the stability of liposomes loaded with negatively charged protein (bovine serum albumin, BSA) during in vitro digestion. Zeta-potential and morphology measurements confirmed that BSA-loaded liposomes were successfully prepared, with an encapsulation efficiency of around 34%. The encapsulated BSA and the integrity of the liposomes remained unchanged with time when the liposomes were digested in a simulated gastric environment, suggesting that the liposomal membrane protected the entrapped BSA from pepsin hydrolysis. BSA-loaded liposomes exhibited lower stability in simulated intestinal fluid, as shown by damaged membranes and the release of free fatty acids. Also, lipolysis kinetics revealed that bile salts and ionic strength could facilitate a high level of free fatty acid release. This work further supplemented our knowledge about the effects of gastrointestinal digestion conditions on liposomal properties and provided valuable information for the design of liposome formulations for the food and health care industries. (C) 2014 Elsevier Ltd. All rights reserved.