PHOTOREVERSIBLE UV‐INACTIVATION OF MESSENGER RNA IN AN INSECT EMBRYO (SMITTIA SPEC., CHIRONOMIDAE, DIPTERA)
PHOTOREVERSIBLE UV‐INACTIVATION OF MESSENGER RNA IN AN INSECT EMBRYO (SMITTIA SPEC., CHIRONOMIDAE, DIPTERA)
复制标题
昆虫胚胎中信使 RNA 的光可逆紫外线灭活（Smittia SPEC.、摇蚊科、双翅目）
DOI：
10.1111/j.1751-1097.1980.tb04052.x
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发表时间：
1980
影响因子：
3.3
通讯作者：
K. Kalthoff
中科院分区：
文献类型：
--
作者：
H. Jäckle;K. Kalthoff
在卵黄内卵裂期间对Smittia胚胎进行UV照射。在这个阶段，细胞核被富含蛋黄的细胞质严重屏蔽，并且不合成可检测量的RNA。如前所述，265、285和295 nm波长的辐照导致生物失活，并在母体RNA中形成嘧啶二聚体（Kalthoff，1976; Jäckie和Kalthoff，1978）。此外，我们观察到对蛋白质合成的显著影响：（1）体内[35 S]-蛋氨酸掺入的总体速率降低至正常速率的一半以下。(2)二维凝胶电泳揭示了一些多肽合成速率的定量变化，以及紫外线照射胚胎中新多肽的出现。(3)在无细胞系统中，来自Smittia胚胎的多聚腺苷酸化RNA的翻译被体内UV照射抑制。(4)在早期胚胎发生过程中，母体多聚腺苷酸化RNA的明显降解在UV照射的胚胎中被延迟。
Smittia embryos were UV‐irradiated during intravitelline cleavage. At this stage, nuclei are heavily shielded by yolk‐rich cytoplasm, and do not synthesize detectable amounts of RNA. Irradiation at 265, 285 and 295 nm wavelength caused biological inactivation, and pyrimidine dimer formation in maternal RNA as described earlier (Kalthoff, 1976; Jäckie and Kalthoff, 1978). In addition, we observed marked effects on protein synthesis: (1) The overall rate of [35S]‐methionine incorporation in vivo was reduced to less than half of the normal rate. (2) Two‐dimensional gel electrophoresis revealed quantitative variations in the synthetic rate of some polypeptides, and the appearance of new ones in UV‐irradiated embryos. (3) Translation of polyadenylated RNA from Smittia embryos in a cell‐free system was inhibited by UV irradiation in vivo. (4) The apparent degradation, during early embryogenesis, of maternal polyadenylated RNA was retarded in UV‐irradiated embryos.