Microarray analysis of the genes induced by tetracycline-regulated expression of NDRF/NeuroD2 in P19 cells
Microarray analysis of the genes induced by tetracycline-regulated expression of NDRF/NeuroD2 in P19 cells
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DOI：
10.1016/j.bbrc.2005.07.093
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发表时间：
2005-09-23
影响因子：
3.1
通讯作者：
Ohkubo, H
中科院分区：
文献类型：
--
作者：
Oda, H;Fushimi, F;Ohkubo, H
NeuroD-related factor（NDRF）/NeuroD 2是一种碱性螺旋-环-螺旋（bHLH）蛋白，在神经元发育中起重要作用。为了阐明NDRF转录网络，我们使用小鼠cDNA微阵列分析结合四环素可调控的表达系统在P19胚胎癌细胞。在NDRF蛋白的存在下，五个基因被鉴定为上调。RNA杂交分析证实，脑脂质结合蛋白（BLBP）和分化抑制因子I（Id 1）基因是NDRF诱导后快速显著上调的5个基因之一。当显性负性形式的NDRF蛋白在维甲酸诱导的P19细胞神经元分化过程中表达时，BLBP基因，而不是Id I基因，被有力地抑制。免疫组织化学分析表明，NDRF和Id I免疫反应观察到一些颗粒细胞的小脑在出生后的时期。这些结果表明，NDRF或其相关的bHLH蛋白可能在这些基因的上游作用于发育中的神经元的一个子集。(c)2005年爱思唯尔公司All rights reserved.
NeuroD-related factor (NDRF)/NeuroD2 is a basic helix-loop-helix (bHLH) protein that plays important roles in neuronal development. To elucidate the NDRF transcription network, we used mouse cDNA microarray analysis combined with a tetracycline-regulatable expression system in P19 embryonal carcinoma cells. Five genes were identified to be up-regulated in the presence of NDRF protein. RNA hybridization analysis confirmed that brain-lipid-binding protein (BLBP) and inhibitor of differentiation I (Id 1) genes were among the five genes that were rapidly and significantly up-regulated after induction of NDRF. When a dominant negative form of NDRF protein was expressed during retinoic acid-induced neuronal differentiation of P19 cells, the BLBP gene, but not the Id I gene, was potently repressed. Immunohistochemical analysis revealed that both NDRF and Id I immunoreactivities were observed in some granule cells of the cerebellum in the postnatal period. These results suggest that NDRF or its related bHLH proteins may act upstream of these genes in a subset of developing neurons. (c) 2005 Elsevier Inc. All rights reserved.