γ-herpesvirus latency is preferentially maintained in splenic germinal center and memory B cells
γ-herpesvirus latency is preferentially maintained in splenic germinal center and memory B cells
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DOI：
10.1084/jem.20020890
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发表时间：
2002-11-18
影响因子：
15.3
通讯作者：
Blackman, MA
中科院分区：
文献类型：
--
作者：
Flaño, E;Kim, IJ;Blackman, MA
伽马疱疹病毒是一种致癌的B细胞淋巴营养病毒，在宿主中建立终身潜伏期。小鼠γ-疱疹病毒68(MHV-68)感染小鼠是一种独特的分析感染不同阶段脾B细胞中伽玛-疱疹病毒潜伏时间的系统。经鼻感染MHV-68后，我们分析了感染后14天的潜伏期的建立，以及感染3个月后脾中不同纯化B细胞亚群的潜伏期的维持。数据表明，MHV-68潜伏期主要存在于生发中心B细胞，而长期潜伏期优先维持在生发中心和记忆B细胞两个不同亚群的同型转换B细胞中。细胞周期分析表明，MHV-68存在于周期和静息同型转换的B细胞中。对病毒基因表达的分析表明，在长期潜伏期间，病毒的裂解和潜伏转录产物在生发中心和记忆B细胞中均有差异表达。总之，这些观察表明，伽玛疱疹病毒利用了脾中的B细胞生命周期。
The gamma-herpesviriises are oncogenic B cell lymphotrophic viruses that establish life-long latency in the host. Murine gamma-herpesvirus 68 (MHV-68) infection of mice represents a unique system for analyzing gamma-herpesvirus latency in splenic B cells at different stages of infection. After intranasal infection with MHV-68 we analyzed the establishment of latency 14 days after infection, and the maintenance of latency 3 months after infection in different purified subpopulations of B cells in the spleen. The data show that MHV-68 latency is mainly established in germinal center B cells and that long-term latency is preferentially maintained in two different subsets of isotype-switched B cells, germinal center and memory B cells. Cell cycle analysis indicates that MHV-68 is located in both cycling and resting isotype-switched B cells. Analysis of viral gene expression showed that both lyric and latent viral transcripts were differentially expressed in germinal center and memory B cells during long-term latency. Together, these observations suggested that gamma-herpesviruses exploit the B cell life cycle in the spleen.