SDF-1 alpha regulates mesendodermal cell migration during frog gastrulation.
SDF-1 alpha regulates mesendodermal cell migration during frog gastrulation.
复制标题
DOI：
--
复制
发表时间：
2007
影响因子：
3.1
通讯作者：
A. Fukui;Toshiyasu Goto;J. Kitamoto;M. Homma;M. Asashima
中科院分区：
文献类型：
--
作者：
A. Fukui;Toshiyasu Goto;J. Kitamoto;M. Homma;M. Asashima
在青蛙原肠胚形成过程中，中内胚层细胞通过内胚层旋转与囊胚腔顶部（BCR）贴合，并向动物极迁移。中内胚层细胞（LEM）的前缘有助于退化边缘区（IMZ）细胞的定向迁移，但这一过程的分子机制尚不清楚。在这里，我们表明 CXCR4/SDF-1 信号传导介导非洲爪蟾胚胎中 LEM 的定向运动。在 IMZ 中检测到 xCXCR4 的表达，并通过 BCR 内表面中的 xSDF-1α 表达进行补充。 xCXCR4 和 xSDF-1alpha 的过度表达导致原肠胚形成缺陷。 xCXCR4 N 末端缺失构建体和 xSDF-1alpha-MO 也抑制原肠胚形成。此外，在 xSDF-1α 存在的情况下，LEM 的外植体向背侧 BCR 迁移，并且 LEM 中 xCXCR4 表达的改变抑制了 LEM 迁移。这些结果表明 CXCR4/SDF-1 信号传导对于原肠胚形成期间大量细胞的迁移是必需的。
During frog gastrulation, mesendodermal cells become apposed to the blastocoel roof (BCR) by endoderm rotation, and migrate towards the animal pole. The leading edge of the mesendodermal cells (LEM) contributes to the directional migration of involuting marginal zone (IMZ) cells, but the molecular mechanism of this process is not well understood. Here we show that CXCR4/SDF-1 signaling mediates the directional movement of the LEM in Xenopus embryos. Expression of xCXCR4 was detected in the IMZ, and was complemented by xSDF-1alpha expression in the inner surface of the BCR. Over-expression of xCXCR4 and xSDF-1alpha caused gastrulation defects. An xCXCR4 N-terminus deletion construct and xSDF-1alpha-MO also inhibited gastrulation. Furthermore, explants of LEM migrate towards the dorsal BCR in the presence of xSDF-1alpha, and altered xCXCR4 expression in the LEM inhibited LEM migration. These results suggest that CXCR4/SDF-1 signaling is necessary for the migrations of massive numbers of cells during gastrulation.