Comprehensive Chemical Characterization of Qingkailing Capsules by Ultra-High-Performance Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Comprehensive Chemical Characterization of Qingkailing Capsules by Ultra-High-Performance Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
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DOI：
10.3390/separations10120588
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发表时间：
2023-12
期刊：
影响因子：
2.6
通讯作者：
Ting Liu;Shu Lin
中科院分区：
文献类型：
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作者：
Ting Liu;Shu Lin
清开灵胶囊是治疗发烧临床疗效显着的经典中药方剂。但清开灵胶囊的化学成分目前尚不清楚。为了获得和表征清开灵胶囊的化学特征，本研究采用快速、准确、灵敏的超高效液相色谱结合傅里叶变换离子回旋共振质谱（UHPLC-FT-ICR-MS）的方法对清开灵胶囊进行全面的化学表征。利用UHPLC的高分离速度和良好的分离效果，准确的质量数据（5 ppm以内）和碎片离子，总共276种化合物被明确或初步鉴定，其中包括67种黄酮及其苷、52种有机酸、75种萜类化合物、23种类固醇、22种苯丙素和37种其他化合物。此次对清开灵胶囊化学成分的全面分析，有助于清开灵胶囊的质量评价，为进一步研究原型成分和体内代谢物以及药理研究提供科学合理的依据，最终促进清开灵胶囊的进一步开发和临床应用的治疗用途。
Qingkailing capsules are a classic traditional Chinese medicine prescription with remarkable clinical effects for the treatment of fevers. However, the chemical components of Qingkailing capsules are still unclear. To obtain and characterize the chemical profile of Qingkailing capsules, the present study applied a rapid, accurate, and sensitive method using ultra-high-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) to perform a comprehensive chemical characterization of Qingkailing capsules. Leveraging the high separation speed and good separation of UHPLC, the accurate mass data (within 5 ppm) and fragment ions, a total of 276 compounds, including 67 flavonoids and their glycosides, 52 organic acids, 75 terpenoids, 23 steroids, 22 phenylpropanoids, and 37 other compounds, were unambiguously or tentatively identified. This comprehensive analysis of the chemical components of Qingkailing capsules contributes to the quality evaluation and provides a scientific and reasonable basis for further study of prototype components and metabolites in vivo and pharmacological research, ultimately facilitating the advancement of Qingkailing capsules for further development and the therapeutic use of Qingkailing capsules in clinical applications.