Mitochondrial DNA loss caused by ethanol in Saccharomyces flor yeasts
Mitochondrial DNA loss caused by ethanol in Saccharomyces flor yeasts
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DOI：
10.1128/aem.63.1.7-12.1997
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发表时间：
1997-01-01
影响因子：
4.4
通讯作者：
Jimenez, J
中科院分区：
文献类型：
--
作者：
Ibeas, JI;Jimenez, J
含酒量超过15%(体积分数)的雪利酒表面生长着酵母，由于乙醇是呼吸缺陷突变的强大诱导剂，这种酒精被认为是酿酒酵母和其他酿酒酵母线粒体基因组高度多样性的来源。Southern印迹分析表明，线粒体DNA(MtDNA)的多态变化是由于线粒体基因组的微小损伤，本工作通过脉冲场凝胶电泳、限制性内切酶分析和Southern印迹分析确定，乙醇诱导的小突变体完全缺乏mtDNA(Rho(0))。没有发现乙醇引起的线粒体基因组的变化，这可以解释在FOR酵母中观察到的mtDNA多态。将两个不同的mtDNA变异体从FLOR酵母转移到实验室菌株后，在这两种情况下，受体菌株的乙醇耐受性都会增加，这表明mtDNA可能受到正选择压力，因为它们具有赋予酒精耐受性的能力。
Saccharomyces flor yeasts proliferate at the surface of sherry wine, which contains over 15% (vol) ethanol, Since ethanol is a powerful inducer of respiration-deficient mutants, this alcohol has been proposed to be the source of the high diversity found in the mitochondrial genomes of flor yeasts and other,vine yeasts. Southern blot analysis suggests that mitochondrial DNA (mtDNA) polymorphic changes are due to minor lesions in the mitochondrial genome, As determined in this work by pulsed-field gel electrophoresis, restriction analysis, and Southern blot analysis, ethanol-induced petite mutants completely lack mtDNA (rho(0)). Ethanol-induced changes in the mitochondrial genome that could explain the observed mtDNA polymorphism in flor yeasts were not found. The transfer of two different mtDNA variants from flor yeasts to a laboratory strain conferred in both cases an increase in ethanol tolerance in the recipient strain, suggesting that mtDNAs are probably subjected to positive selection pressure concerning their ability to confer ethanol tolerance.