Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies
Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies
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DOI：
10.1016/j.jep.2003.08.008
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发表时间：
2003-12-01
影响因子：
5.4
通讯作者：
Thongnoi, W
中科院分区：
文献类型：
--
作者：
Ingkaninan, K;Temkitthawon, P;Thongnoi, W
乙酰胆碱酯酶（AChE）抑制剂已被用作阿尔茨海默病的对症治疗药物。为了寻找新的乙酰胆碱酯酶抑制剂，32植物用于泰国传统的振兴和神经紧张的补救措施收集。用Ellman比色法在96孔微孔板中测定植物甲醇提取物的AChE抑制活性。结果表明：铁线蕨根的甲醇提取物具有较好的抗肿瘤活性。和Tabernaemontana divaricata（L.）R.Br.来自罗姆。& Schutt.浓度为0.1mg/ml时，AChE活性抑制率达90%以上。在相同浓度下，胡椒种子，紫铆树的根皮。和决明子（Cassia fistula L.提取物对乙酰胆碱酯酶的抑制率为50-65%。其余提取物对乙酰胆碱酯酶的抑制率均在50%以下。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
Acetylcholinesterase (AChE) inhibitor has been used as a drug for the symptomatic treatment of Alzheimer's disease. In order to search for new AChE inhibitors, 32 plants used in Thai traditional rejuvenating and neurotonic remedies were collected. The plant methanolic extracts were tested for AChE inhibitory activity using Ellman's colorimetric method in 96-welled microplates. The results showed that the methanolic extracts from roots of Stephania suberosa Forman. and Tabernaemontana divaricata (L.) R.Br. ex Roem. & Schutt. at concentration of 0.1 mg/ml inhibited more than 90% of AChE activity. At the same concentration, four extracts, i.e. stems of Piper interruptum Opiz., seeds of Piper nigrum L., rootbarks of Butea superba Roxb. and roots of Cassia fistula L. extracts showed 50-65% inhibitory activity on AChE. The rest of the extracts showed the AChE inhibitory activity below 50%. (C) 2003 Elsevier Ireland Ltd. All rights reserved.