Overexpression of Alternative Oxidase Gene Confers Aluminum Tolerance by Altering the Respiratory Capacity and the Response to Oxidative Stress in Tobacco Cells
Overexpression of Alternative Oxidase Gene Confers Aluminum Tolerance by Altering the Respiratory Capacity and the Response to Oxidative Stress in Tobacco Cells
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DOI：
10.1007/s12033-012-9595-7
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发表时间：
2013-06-01
影响因子：
2.6
通讯作者：
Matsumoto, Hideaki
中科院分区：
文献类型：
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作者：
Panda, Sanjib Kumar;Sahoo, Lingaraj;Matsumoto, Hideaki
铝胁迫抑制植物线粒体呼吸，产生活性氧。线粒体交替氧化酶（AOX）将呼吸作用与线粒体ATP产生解偶联，并可能通过防止ROS的过度积累来改善植物在铝胁迫下的表现。研究了铝胁迫下烟草（SL，ALT301）线粒体和全细胞的呼吸变化和活性氧（ROS）的产生。铝胁迫下，ALT 301的线粒体和全细胞中AOX途径的活性均高于细胞色素途径。AOX1在研究时显示，在应激下ALT 301中的AOX1表达高于SL细胞。为了研究烟草AOX基因在铝胁迫下的功能，我们将花椰菜花叶病毒（CaMV）35 S启动子控制下表达的NtAOX 1导入敏感烟草（SL），获得转化烟草细胞系。细胞系铝胁迫对内源AOX 1表达和AOX蛋白表达的促进作用均表现为转化细胞系> ALT 301>野生型（SL）。在铝胁迫下，AOX1转化细胞的显著特征是呼吸抑制降低，ROS产生减少，生长能力增强。这些结果表明，AOX在烟草细胞耐铝胁迫中起着关键作用，其增强了呼吸能力，降低了线粒体氧化应激负荷，提高了细胞的生长能力。
Aluminum (Al) stress represses mitochondrial respiration and produces reactive oxygen species (ROS) in plants. Mitochondrial alternative oxidase (AOX) uncouples respiration from mitochondrial ATP production and may improve plant performance under Al stress by preventing excess accumulation of ROS. We tested respiratory changes and ROS production in isolated mitochondria and whole cell of tobacco (SL, ALT 301) under Al stress. Higher capacities of AOX pathways relative to cytochrome pathways were observed in both isolated mitochondria and whole cells of ALT301 under Al stress. AOX1 when studied showed higher AOX1 expression in ALT 301 than SL cells under stress. In order to study the function of tobacco AOX gene under Al stress, we produced transformed tobacco cell lines by introducing NtAOX1 expressed under the control of the cauliflower mosaic virus (CaMV) 35 S promoter in sensitive (SL) Nicotiana tabacum L. cell lines. The enhancement of endogenous AOX1 expression and AOX protein with or without Al stress was in the order of transformed tobacco cell lines > ALT301 > wild type (SL). A decreased respiratory inhibition and reduced ROS production with a better growth capability were the significant features that characterized AOX1 transformed cell lines under Al stress. These results demonstrated that AOX plays a critical role in Al stress tolerance with an enhanced respiratory capacity, reducing mitochondrial oxidative stress burden and improving the growth capability in tobacco cells.