Receptors for hyaluronate on the surface of parent and virus-transformed cell lines: binding and aggregation studies.
Receptors for hyaluronate on the surface of parent and virus-transformed cell lines: binding and aggregation studies.
复制标题
亲本和病毒转化细胞系表面的透明质酸受体：结合和聚集研究。
DOI：
10.1016/0014-4827(81)90248-2
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发表时间：
1981
影响因子：
3.7
通讯作者：
Toole,BP
中科院分区：
文献类型：
--
作者：
Underhill,CB;Toole,BP
比较了亲本和病毒转化的两组细胞系(3T3vsSV-3T3；BHK vsPY-BHK)的二价键非依赖性聚集的程度，以前已经证明这种聚集依赖于内源性透明质酸与细胞表面特定受体的相互作用。在生理离子强度条件下，病毒转化的细胞系(SV-3T3和PY-BHK)有大量透明质酸酶抑制的聚集，而亲本细胞(3T3和BHK)则没有。然而，当同样的实验在高离子强度溶液(0.5M氯化钠)中进行时，所有细胞系中都检测到透明质酸酶抑制聚集。不同细胞系之间聚集的差异也反映在[~3H]透明质酸的结合上。在生理盐水中，病毒转化的细胞与更多的透明质酸结合(高Bmax)，与未转化的细胞相比具有更高的亲和力(低kd)。当离子强度增加到0.5M时，各细胞系与[~3H]透明质酸的结合量增加，但各细胞系之间的相对差异仍然存在。这些结果表明，细胞结合透明质酸的能力的差异可以部分解释亲本细胞和病毒转化细胞在聚集能力方面的差异。
Two sets of parent and virus-transformed cell lines (3T3 vs SV-3T3; BHK vs PY-BHK) were compared with respect to the extent of divalentcation independent aggregation which previously has been shown to depend upon the interaction of endogenous hyaluronate with specific receptors on the cell surface. When measured under conditions of physiological ionic strength, a significant amount of hyaluronidase-inhibitable aggregation was found in the virus-transformed cell lines (SV-3T3 and PY-BHK) but not in their parent counterparts (3T3 and BHK). However, when the same experiment was performed in a high ionic strength solution (0.5 M NaCl), the hyaluronidase inhibitable aggregation was detected in all of the cell lines. The differences in the aggregation between the various cell lines was also reflected in the binding of [3H]hyaluronate. In physiological saline, the virus-transformed cells bound greater amounts of hyaluronate (higherBmax) with a greater affinity (lowerkd) than did their untransformed counterparts. Increasing the ionic strength to 0.5 M NaCl increased the binding of [3H]hyaluronate by each cell line; however, the relative differences between the cell lines remained. These results indicate that variations in the ability of the cells to bind hyaluronate can partially account for the differences between the parent and the virus-transformed cells with respect to their ability to aggregate.