RGA protein associates with a TRPV ion channel during biosynthesis and trafficking
RGA protein associates with a TRPV ion channel during biosynthesis and trafficking
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DOI：
10.1002/jcb.10775
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发表时间：
2004-03-01
影响因子：
4
通讯作者：
Turner, H
中科院分区：
文献类型：
--
作者：
Barnhill, JC;Stokes, AJ;Turner, H
TRPV 离子通道可转换一系列温度刺激。我们提出，对 TRPV2 产生的蛋白质-蛋白质相互作用的分析可能有助于深入了解围绕该通道的关键问题。这些问题包括 TRPV2 在非感觉组织中的潜在功能意义、参与转导其激活信号的分子以及调节其运输到细胞表面的机制。在这里，我们描述了 TRPV2 通道与 RGA 基因产物的相互作用。 RGA 是一种四跨膜结构域、细胞内定位蛋白。 RGA 与大鼠肥大细胞系中的 TRPV2 相关，而大鼠肥大细胞系是这两种蛋白质的天然环境。 TRPV2 和 RGA 之间的相互作用是短暂的并且发生在细胞内。 RGA 不伴随 TRPV2 到达细胞表面。 TRPV2/RGA 复合物的形成取决于细胞糖基化事件，表明 RGA 可能在离子通道蛋白成熟过程中对 TRPV2 发挥伴侣或靶向作用。这些数据记录了 TRPV2 的新型蛋白质-蛋白质相互作用，并为未来研究 RGA 对 TRPV2 功能的潜在调节贡献奠定了基础。 (C) 2004 Wiley-Liss, Inc.
TRPV ion channels transduce a range of temperature stimuli. We proposed that analysis of the protein-protein interactions made by TRPV2 might give insight into the key issues surrounding this channel. These issues include the potential functional significance of TRPV2 in non-sensory tissues, the molecules involved in transducing its activation signal(s) and the mechanism by which its trafficking to the cell surface is regulated. Here we describe the interaction of TRPV2 channel with the RGA gene product. RGA is a four-transmembrane domain, intracellularly localized protein. RGA associates with TRPV2 in a rat mast cell line that is a native context for both proteins. The interaction between TRPV2 and RGA is transient and occurs intracellularly. RGA does not accompany TRPV2 to the cell surface. Formation of the TRPV2/RGA complex is dependent upon a cellular glycosylation event, suggesting that RGA may play a chaperone or targeting role for TRPV2 during the maturation of the ion channel protein. These data record a novel protein-protein interaction for TRPV2 and provide a foundation for future study of the potential regulatory contribution of RGA to TRPV2 function. (C) 2004 Wiley-Liss, Inc.