A novel defensin-like peptide from salivary glands of the hard tick, Haemaphysalis longicornis
A novel defensin-like peptide from salivary glands of the hard tick, Haemaphysalis longicornis
复制标题
DOI：
10.1002/pro.317
复制
发表时间：
2010-03-01
期刊：
影响因子：
8
通讯作者：
Yu, Haining
中科院分区：
文献类型：
--
作者：
Lu, Xiangyun;Chen, Qiaolin;Yu, Haining
采用10-kDa截留分子量的Centriprep过滤器和反相高效液相色谱法（RP-HPLC），从长角血蜱（Haemaphysalis longicornis）唾液腺中分离得到一种新的防御素样抗菌肽，命名为长角血蜱素（Longicornsin）。经Edman降解法测定其氨基酸序列为DFGCGQGMIFMCQRRCMRLYPGSTGFCRGFRCMCDTHIPLRPPFMVG。通过cDNA文库筛选，克隆了长角蛋白的cDNA。从cDNA序列预测的蛋白质由78个氨基酸组成，包括一个成熟的长角蛋白。经BLAST检索，与其它蜱类防御素样肽序列相似。与大多数其他蜱防御素样肽不同，长角蛋白具有C-末端延伸。纯化的天牛素对细菌和真菌具有较强的抗菌活性。有趣的是，它甚至对耐药微生物和幽门螺杆菌显示出很强的抗菌能力。本研究结果表明，长角蛋白是一个潜在的候选新的抗菌药物设计。
A novel defensin-like antimicrobial peptide named longicornsin was isolated from the salivary glands of the hard tick, Haemaphysalis longicornis, using a 10-kDa cut-off Centriprep filter and reversed-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as DFGCGQGMIFMCQRRCMRLYPGSTGFCRGFRCMCDTHIPLRPPFMVG by Edman degradation. The cDNA encoding longicornsin was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 78 amino acids including a mature longicornsin. It showed similarity with defensin-like peptides from other ticks by BLAST search. Different from most other tick defensin-like peptides, longicornsin had a C-terminal extension. Purified longicornsin exerted potent antimicrobial activities against bacteria and fungi. Interestingly, it even showed strong antimicrobial ability against drug-resistant microorganisms and Helicobacter pylori. The results of this study indicated that longicornsin is a potential candidate for novel antimicrobial drug design.