Gram-Positive Marine Bacteria as a Potential Resource for the Discovery of Quorum Sensing Inhibitors
Gram-Positive Marine Bacteria as a Potential Resource for the Discovery of Quorum Sensing Inhibitors
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DOI：
10.1007/s10126-010-9334-7
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发表时间：
2011-08-01
影响因子：
3
通讯作者：
Rowley, David C.
中科院分区：
文献类型：
--
作者：
Teasdale, Margaret E.;Donovan, Kellye A.;Rowley, David C.
细菌群体感应抑制剂已被提出作为治疗某些细菌疾病的潜在新疗法。我们最近报道了一种海洋盐生盐芽孢杆菌分离物，其分泌的次级代谢产物能够淬灭几种革兰氏阴性报告菌株中的群体感应表型。为了研究这些化合物在海洋细菌环境中的生产有多普遍，对来自不同生境的332株革兰氏阳性菌株进行了检测，以检测其干扰哈氏弧菌生物发光（一种细胞信号调节表型）的能力。采用快速测定方法，其中环境分离株与报告菌株一起繁殖。“活性物”定义为干扰生物发光而没有可见的细胞杀伤作用（抗生素活性）的细菌。在测定中，共有49株细菌分离株干扰生物发光的产生。从活性分离物的培养物中产生代谢产物提取物，并且28个再现了对哈维氏弧菌的生物发光抑制。在这28种提取物中，有5种提取物还抑制了紫色色杆菌的紫色素生产。化学研究表明，苯乙酰胺和环二肽是两种类型的次级代谢产物负责观察到的活动。活性细菌分离物主要属于芽孢杆菌属或盐芽孢杆菌属。结果表明，革兰氏阳性海洋细菌是值得进一步研究的群体感应拮抗剂的发现。
Inhibitors of bacterial quorum sensing have been proposed as potentially novel therapeutics for the treatment of certain bacterial diseases. We recently reported a marine Halobacillus salinus isolate that secretes secondary metabolites capable of quenching quorum sensing phenotypes in several Gram-negative reporter strains. To investigate how widespread the production of such compounds may be in the marine bacterial environment, 332 Gram-positive isolates from diverse habitats were tested for their ability to interfere with Vibrio harveyi bioluminescence, a cell signaling-regulated phenotype. Rapid assay methods were employed where environmental isolates were propagated alongside the reporter strain. "Actives" were defined as bacteria that interfered with bioluminescence without visible cell-killing effects (antibiotic activity). A total of 49 bacterial isolates interfered with bioluminescence production in the assays. Metabolite extracts were generated from cultures of the active isolates, and 28 reproduced the bioluminescence inhibition against V. harveyi. Of those 28, five extracts additionally inhibited violacein production by Chromobacterium violaceum. Chemical investigations revealed that phenethylamides and a cyclic dipeptide are two types of secondary metabolites responsible for the observed activities. The active bacterial isolates belonged primarily to either the genus Bacillus or Halobacillus. The results suggest that Gram-positive marine bacteria are worthy of further investigation for the discovery of quorum sensing antagonists.