Cross-species sperm-FISH assays for chemical testing and assessing paternal risk for chromosomally abnormal pregnancies.
Cross-species sperm-FISH assays for chemical testing and assessing paternal risk for chromosomally abnormal pregnancies.
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跨物种精子 FISH 检测用于化学检测和评估染色体异常妊娠的父亲风险。
DOI：
10.1002/em.20121
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Marchetti,Francesco
中科院分区：
文献类型：
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作者：
Wyrobek,AndrewJ;Schmid,ThomasE;Marchetti,Francesco
父亲和母亲一样，可以将不利于正常发育和健康生活的遗传缺陷传给后代。流行病学研究已经确定了几种父亲暴露与异常生殖结果之间的关联，但这些类型的研究本身就复杂且昂贵，并且对父亲对异常生殖结果的贡献的风险因素仍然知之甚少。已经开发了几种灵敏的方法来直接检测精子中的突变和染色体损伤。这些检测是潜在的生物指示剂的父亲的风险因素不育，自然流产，非整倍体综合征，遗传疾病的儿童。在这些方法中，荧光原位杂交（FISH）已适用于检测越来越多的物种（包括人类和啮齿动物）精子中染色体数目和结构异常。精子FISH已经确定了几个潜在的父亲风险因素，如年龄，药物，生活方式和各种环境/职业暴露。在这里，我们总结了这些精子-FISH检测的开发和使用现状，并提出了优先考虑化学试剂进行流行病学调查的策略，以评估父亲异常生殖结果的风险。Environ.摩尔诱变剂，2005. 2005年出版Wiley利斯公司
The father, like the mother, can transmit genetic defects to his offspring that are detrimental for normal development and a healthy life. Epidemiological studies have identified associations between several paternal exposures and abnormal reproductive outcomes, but these types of studies are inherently complex and expensive, and the risk factors for the paternal contribution to abnormal reproductive outcomes remain poorly understood. Several sensitive methods have been developed for detecting mutations and chromosomal damage directly in sperm. These assays are potential bioindicators for paternal risk factors for infertility, spontaneous abortions, aneuploidy syndromes, and genetic diseases in children. Among these methods, fluorescence in situ hybridization (FISH) has been adapted for the detection of numerical and structural chromosomal abnormalities in the sperm of an expanding number of species, including humans and rodents. Sperm FISH has identified several potential paternal risk factors such as age, drugs, lifestyles, and various environmental/occupational exposures. Here, we summarize the status of the development and usage of these sperm‐FISH assays and suggest strategies for prioritizing chemical agents for epidemiological investigations to assess paternal risk for abnormal reproductive outcome. Environ. Mol. Mutagen., 2005. Published 2005 Wiley‐Liss, Inc.