DNA protection mechanisms are not involved in the radioresistance of the hyper thermophilic archaea Pyrococcus abyssi and P-furiosus
DNA protection mechanisms are not involved in the radioresistance of the hyper thermophilic archaea Pyrococcus abyssi and P-furiosus
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DOI：
10.1007/s004380100520
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发表时间：
2001-09-01
影响因子：
3.1
通讯作者：
Forterre, P
中科院分区：
文献类型：
--
作者：
Gérard, E;Jolivet, E;Forterre, P
热球菌属的嗜热古生菌对伽马辐射具有抵抗力，这表明在这些生物中存在有效的DNA修复机制。为了确定保护机制是否也可能与这种辐射抗性有关，我们以大肠杆菌和耐辐射细菌耐辐射球菌为对照，估计了两种热球菌基因组DNA中伽马辐射引起的DNA双链断裂的线性密度。在相似的厌氧条件下进行辐照时，四种微生物双链断裂的线密度基本相同，表明在热球菌种中不存在特定的DNA保护机制。利用一维和二维凝胶电泳法，我们比较了已经或没有暴露于伽马射线的深海热球菌和狂热杆菌细胞的蛋白质图谱。在这两个物种的DNA损伤后，我们没有检测到任何显著的蛋白质诱导。
Hyperthermophilic archaea of the genus Pyrococcus are resistant to gamma radiation, suggesting that efficient mechanisms for DNA repair exist in these organisms. To determine whether protective mechanisms might also be implicated in this radioresistance, we have estimated the linear density of DNA double-stranded breaks caused by gamma irradiation in the genomic DNA of two Pyrococcus species, using Escherichia coli and the radioresistant bacterium Deinococcus radiodurans as controls. The linear density of double-stranded breaks was essentially the same in all four microorganisms when irradiation was carried under similar anaerobic conditions, indicating that no specific DNA protection mechanisms exist in Pyrococcus species. Using one- and two-dimensional gel electrophoresis we compared the protein patterns from Pyrococcus abyssi and P. furiosus cells that had or had not been exposed to gamma rays. We did not detect any significant protein induction following DNA damage in either species.