Experimental silicosis does not aggravate collagen-induced arthritis in mice
Experimental silicosis does not aggravate collagen-induced arthritis in mice
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DOI：
10.1186/s12952-017-0071-6
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发表时间：
2017-03-13
期刊：
影响因子：
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通讯作者：
Mueller-Hilke, Brigitte
中科院分区：
文献类型：
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作者：
Engelmann, Robby;Mueller-Hilke, Brigitte
目的：探讨慢性肺部炎症对胶原性关节炎小鼠发病及严重程度的影响。方法：采用二氧化硅微粒鼻腔给药诱导矽肺模型。一周后开始II型胶原免疫，加强免疫六周后处死小鼠。结果：与PBS对照组相比，染矽尘组小鼠的肺部炎症明显增加，肺泡灌洗液(BAL)细胞计数显著增加，这是由于巨噬细胞和粒细胞的数量增加所致。肺部炎症与PAD2和PAD4表达升高无关，但二氧化硅处理组动物的ACCP血清效价显著高于对照组。结论：矽肺慢性肺部炎症不会加重胶原性关节炎小鼠的发病。
Objectives: To investigate the effect of chronic lung inflammation on the incidence and severity of collagen-induced arthritis in mice.Methods: Chronic lung inflammation in the form of silicosis was induced via intranasal application of silica particles. Immunization with collagen Type II commenced one week later and mice were sacrificed six weeks after booster immunization. Thereafter, silicosis was confirmed via flow cytometry and arthritis was evaluated performing knee and paw histology.Results: Pronounced lung inflammation in the silica-treated compared to PBS-treated control mice was demonstrated by significantly elevated broncho-alveolar lavage (BAL) cell count, attributable to increased numbers of macrophages and granulocytes. Inflammation in the lungs was not associated with elevated PAD2 and PAD4 expression, yet silica treated animals had significantly higher aCCP serum titers. However, lung inflammation did not lead to an increase in the incidence of arthritis, nor did it exacerbate the macroscopic or histologic joint scores.Conclusions: Chronic lung inflammation resulting from silicosis does not aggravate collagen-induced arthritis in mice.