Assessment and comparison of putative amine receptor complement/diversity in the brain and eyestalk ganglia of the lobster, Homarus americanus
Assessment and comparison of putative amine receptor complement/diversity in the brain and eyestalk ganglia of the lobster, Homarus americanus
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DOI：
10.1007/s10158-020-0239-5
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发表时间：
2020-03-26
影响因子：
--
通讯作者：
Dickinson, Patsy S.
中科院分区：
文献类型：
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作者：
Christie, Andrew E.;Hull, J. Joe;Dickinson, Patsy S.
在十足动物中，多巴胺、八足胺、血清素和组胺作为局部释放/激素传递的生理/行为调节剂发挥作用。虽然胺在十足动物中所扮演的功能角色已经被广泛研究，但对其胺受体的身份/多样性知之甚少。最近，一个美洲Homarus americanus混合神经系统转录组被用来鉴定该物种中可能的神经元胺受体。虽然鉴定了许多受体，但有些受体是片段的，没有发现剪接/其他变异的证据。在这里，先前预测的蛋白质被用来搜索大脑和眼柄神经节特异性转录组，以评估/比较龙虾神经系统这些部分的胺受体补体。所有先前鉴定的受体都从脑和/或眼柄神经节转录组中重新鉴定，即多巴胺α -1， β -1和α -2 （Homam-DA α 2R）受体，章鱼胺α (Homam-Oct α R), β -1, β -2, β -3， β -4和章鱼胺-酪胺（Homam-OTR-I）受体，血清素1a型，1b型（Homam-5HTR1B）， 2b型和7型受体；以及组胺1型（Homam-HA1R）、2型、3型和4型受体。对于许多先前的部分蛋白，全长受体是从脑和/或眼柄神经节转录物中推断出来的，即Homam-DA α 2R， Homam-Oct α R， homam - otr - 1和Homam-5HTR1B。此外，还发现了新的多巴胺/表皮甾体、章鱼胺α -2和OTR受体，后者为Homam-OTR-II，被认为是Homam-OTR-I的类似物。最后，在许多受体中发现了剪接/其他变异的证据，包括一些装配特异性的证据，例如脑特异性的homam - otr - 1变异和眼柄神经节特异性的Homam-HA1R变异。为了增加转录组衍生序列的可信度，使用RT-PCR克隆了一个受体子集。这些数据补充/增强了先前报道的数据，提供了龙虾神经系统中胺受体补体/多样性的更完整的图像。
In decapods, dopamine, octopamine, serotonin, and histamine function as locally released/hormonally delivered modulators of physiology/behavior. Although the functional roles played by amines in decapods have been examined extensively, little is known about the identity/diversity of their amine receptors. Recently, a Homarus americanus mixed nervous system transcriptome was used to identify putative neuronal amine receptors in this species. While many receptors were identified, some were fragmentary, and no evidence of splice/other variants was found. Here, the previously predicted proteins were used to search brain- and eyestalk ganglia-specific transcriptomes to assess/compare amine receptor complements in these portions of the lobster nervous system. All previously identified receptors were reidentified from the brain and/or eyestalk ganglia transcriptomes, i.e., dopamine alpha-1, beta-1, and alpha-2 (Homam-DA alpha 2R) receptors, octopamine alpha (Homam-Oct alpha R), beta-1, beta-2, beta-3, beta-4, and octopamine-tyramine (Homam-OTR-I) receptors, serotonin type-1A, type-1B (Homam-5HTR1B), type-2B, and type-7 receptors; and histamine type-1 (Homam-HA1R), type-2, type-3, and type-4 receptors. For many previously partial proteins, full-length receptors were deduced from brain and/or eyestalk ganglia transcripts, i.e., Homam-DA alpha 2R, Homam-Oct alpha R, Homam-OTR-I, and Homam-5HTR1B. In addition, novel dopamine/ecdysteroid, octopamine alpha-2, and OTR receptors were discovered, the latter, Homam-OTR-II, being a putative paralog of Homam-OTR-I. Finally, evidence for splice/other variants was found for many receptors, including evidence for some being assembly-specific, e.g., a brain-specific Homam-OTR-I variant and an eyestalk ganglia-specific Homam-HA1R variant. To increase confidence in the transcriptome-derived sequences, a subset of receptors was cloned using RT-PCR. These data complement/augment those reported previously, providing a more complete picture of amine receptor complement/diversity in the lobster nervous system.