Regulated trafficking of the MSP/Eph receptor during oocyte meiotic maturation in C-elegans
Regulated trafficking of the MSP/Eph receptor during oocyte meiotic maturation in C-elegans
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DOI：
10.1016/j.cub.2008.04.043
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发表时间：
2008-05-20
期刊：
影响因子：
9.2
通讯作者：
Greenstein, David
中科院分区：
文献类型：
--
作者：
Cheng, Hua;Govindan, J. Amaranath;Greenstein, David
背景：在C.在线虫中，精子感应机制调节卵母细胞减数分裂成熟和排卵，紧密协调精子可用性和胚胎生产;精子释放主要精子蛋白（MSP）信号，触发减数分裂恢复。减数分裂阻滞依赖于卵母细胞VAB-1 MSP/Eph受体和体细胞G蛋白信号传导的平行功能。MSP促进减数分裂成熟，通过拮抗Eph受体信号和抵消抑制性输入从性腺鞘cells.Results：在这里，我们提出的证据表明，在没有MSP配体的情况下，VAB-1 Eph受体抑制减数分裂成熟，而无论是在或在运输到内吞再循环室。VAB-1：：GFP定位于RAB-11阳性内吞再循环区室不依赖于肝配蛋白，但被MSP信号传导拮抗。卵母细胞减数分裂成熟的两种负调节因子DAB-1 /Disabled和RAN-1与VAB-1受体相互作用，并且在不存在MSP或精子的情况下（下文称为MSP/精子），VAB-1受体在内吞再循环隔室中积累是必需的。内体再循环调节因子rme-1或rab-11.1的失活导致在MSP/精子存在下减数分裂成熟率的vab-1依赖性降低。此外，我们表明，Gas信号在性腺鞘细胞，这是必要的减数分裂成熟的MSP/精子的存在下，影响VAB-1：：GFP运输在卵母细胞。结论：调节内吞运输的VAB-1 MSP/Eph受体有助于控制卵母细胞减数分裂成熟在C。优美的Eph受体在其他系统中的运输可能受到保守蛋白DAB-1/Disabled和RAN-1以及与邻近细胞中G蛋白信号传导的串扰的影响。
Background: In C. elegans, a sperm-sensing mechanism regulates oocyte meiotic maturation and ovulation, tightly coordinating sperm availability and embryo production; sperm release the major sperm protein (MSP) signal to trigger meiotic resumption. Meiotic arrest depends on the parallel function of the oocyte VAB-1 MSP/Eph receptor and somatic G protein signaling. MSP promotes meiotic maturation by antagonizing Eph receptor signaling and counteracting inhibitory inputs from the gonadal sheath cells.Results: Here, we present evidence suggesting that in the absence of the MSP ligand, the VAB-1 Eph receptor inhibits meiotic maturation while either in or in transit to the endocytic-recycling compartment. VAB-1::GFP localization to the RAB-11-positive endocytic-recycling compartment is independent of ephrins but is antagonized by MSP signaling. Two negative regulators of oocyte meiotic maturation, DAB-1 /Disabled and RAN-1, interact with the VAB-1 receptor and are required for its accumulation in the endocytic-recycling compartment in the absence of MSP or sperm (hereafter referred to as MSP/sperm). Inactivation of the endosomal recycling regulators rme-1 or rab-11.1 causes a vab-1-dependent reduction in the meiotic-maturation rate in the presence of MSP/sperm. Further, we show that Gas signaling in the gonadal sheath cells, which is required for meiotic maturation in the presence of MSP/sperm, affects VAB-1::GFP trafficking in oocytes.Conclusions: Regulated endocytic trafficking of the VAB-1 MSP/Eph receptor contributes to the control of oocyte meiotic maturation in C. elegans. Eph receptor trafficking in other systems may be influenced by the conserved proteins DAB-1/Disabled and RAN-1 and by crosstalk with G protein signaling in neighboring cells.