A NERVE GROWTH-FACTOR INDUCED GENE ENCODES A POSSIBLE TRANSCRIPTIONAL REGULATORY FACTOR
A NERVE GROWTH-FACTOR INDUCED GENE ENCODES A POSSIBLE TRANSCRIPTIONAL REGULATORY FACTOR
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DOI：
10.1126/science.3672127
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发表时间：
1987-11-06
期刊：
影响因子：
56.9
通讯作者：
MILBRANDT, J
中科院分区：
文献类型：
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作者：
MILBRANDT, J
神经生长因子（NGF）是一种促进交感神经节和感觉神经节神经纤维生长的营养剂。这种激素刺激的神经元分化在神经生长因子反应细胞系PC 12中进行了检查。应用差异杂交技术筛选经神经生长因子和放线菌酮处理的PC 12细胞的互补DNA文库。发现一个由NGF快速诱导的互补DNA克隆具有预测与转录调节蛋白同源的54千道尔顿蛋白的核苷酸序列。该克隆NGFI-A含有非洲爪蟾TFIIIA和其他DNA结合蛋白中存在的28- 30氨基酸“锌指”结构域的三个串联重复拷贝。它还含有另一个高度保守的单位，由8个氨基酸组成，重复至少11次。NGFI-A基因在成年大鼠的脑、肺和上级颈神经节中以相对高的水平表达。
Nerve growth factor (NGF) is a trophic agent that promotes the outgrowth of nerve fibers from sympathetic and sensory ganglia. The neuronal differentiation stimulated by this hormone was examined in the NGF-responsive cell line PC12. Differential hybridization was used to screen a complementary DNA library constructed from PC12 cells treated with NGF and cycloheximide. One of the complementary DNA clones that was rapidly induced by NGF was found to have a nucleotide sequence that predicts a 54-kilodalton protein with homology to transcriptional regulatory proteins. This clone, NGFI-A, contains three tandemly repeated copies of the 28- to 30-amino acid "zinc finger" domain present in Xenopus laevis TFIIIA and other DNA-binding proteins. It also contains another highly conserved unit of eight amino acids that is repeated at least 11 times. The NGFI-A gene is expressed at relatively high levels in the brain, lung, and superior cervical ganglion of the adult rat.