Single cell genome sequencing.

Single cell genome sequencing.
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单细胞基因组测序。

DOI:
10.1016/j.copbio.2011.11.018
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发表时间:
2012-06
影响因子:
7.7
通讯作者:
Singh, Anup K.
Singh, Anup K.
中科院分区:
工程技术1区
文献类型:
--
作者:
Yilmaz, Suzan;Singh, Anup K.

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单细胞的全基因组扩增和下一代测序已经成为研究占所有环境微生物90- 99%的未培养微生物的有力方法。单细胞测序不仅可以鉴定微生物,还可以将功能与物种联系起来,这是宏基因组技术无法实现的壮举。此外,它还可以分析在基于社区的分析中可能遗漏的低丰度物种。它也被证明是非常有用的补充宏基因组学的组装和装箱的单基因组。随着更便宜和更高通量测序技术的出现,预计单细胞测序将成为研究微生物群落基因组和转录组的标准工具。
Whole genome amplification and next-generation sequencing of single cells has become a powerful approach for studying uncultivated microorganisms that represent 90–99 % of all environmental microbes. Single cell sequencing enables not only the identification of microbes but also linking of functions to species, a feat not achievable by metagenomic techniques. Moreover, it allows the analysis of low abundance species that may be missed in community-based analyses. It has also proved very useful in complementing metagenomics in the assembly and binning of single genomes. With the advent of drastically cheaper and higher throughput sequencing technologies, it is expected that single cell sequencing will become a standard tool in studying the genome and transcriptome of microbial communities.
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