Mapping structural changes in breast tissue disease using x-ray scattering.
Mapping structural changes in breast tissue disease using x-ray scattering.
复制标题
使用 X 射线散射绘制乳腺组织疾病的结构变化。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
R. Lewis
中科院分区：
文献类型：
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作者：
S. Sidhu;K. Siu;G. Falzon;S. Hart;J. Fox;R. Lewis
浸润性癌在整个乳腺组织中的扩散被认为发生在超分子水平，超出了标准组织病理学鉴定的范围。小角X射线散射（SAXS）能够以数十至数百纳米的尺度表征乳房中发现的胶原蛋白和组织的结构特性。五十六例接受广泛局部切除术或乳房切除术的患者，在距肿瘤原发部位6 cm处，沿沿着两个垂直轴，以2 cm间隔对切除肿块进行组织活检样本分析。两个SAXS参数，积分无定形散射和三阶胶原轴向间距，显示中心（0 cm）和远端组织（距离原发性病变2，4或6 cm）之间的显着差异。没有证据表明这两个参数在乳房上的方向趋势（乳头的上级、下级或侧面）。这两个变量在二维网格上的映射显示出与独立组织病理学诊断的良好匹配。这些结果表明，小角X射线散射可能是能够确定的领域的入侵或定向传播的疾病，以及提供更多的信息，在超分子水平，以帮助组织诊断。
Spread of invasive carcinoma throughout breast tissue is believed to occur at supramolecular levels, beyond the range of standard histopathology identification. Small angle x-ray scattering (SAXS) is capable of characterizing the structural properties of collagen and tissue found in the breast at the scale of tens to hundreds of nanometers. Fifty-six patients who were treated with wide-local excision or mastectomy had tissue biopsy samples analyzed at 2 cm intervals along two perpendicular axes over their excised mass, up to 6 cm away from the primary site of the tumor. Two SAXS parameters, the integrated amorphous scatter and the third order collagen axial d spacing, showed significant differences between the center (0 cm) and distant tissues (2, 4, or 6 cm from the primary lesion). There was no evidence of directional trends (superior, inferior, or lateral sides of the nipple) of these two parameters over the breast. Mapping of these two variables over a two-dimensional grid showed good matching with independent histopathology diagnosis. These results suggest that SAXS may be capable of identifying areas of invasion or directional spread of disease as well as providing more information at the supramolecular level for aiding tissue diagnosis.