Flow cytometric evidence for endopolyploidization in cabbage (Brassica oleracea L.) flowers
Flow cytometric evidence for endopolyploidization in cabbage (Brassica oleracea L.) flowers
复制标题
甘蓝花内多倍化的流式细胞术证据
DOI：
10.1007/s004970100066
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发表时间：
2001
影响因子：
--
通讯作者：
Y. Kimura
中科院分区：
文献类型：
--
作者：
N. Kudo;Y. Kimura
甘蓝（Brassica oleraceaL.）揭示了广泛的核内复制，导致组织中含有具有多倍性水平的细胞（也称为“核内多倍性”）。在成熟的花中观察到与2C、4C、8C、16C、32C和64C相对应的单倍体核基因组补体（1C）的倍数。具有不同倍性水平的细胞的分布是组织特异性的，并且是发育阶段的特征。幼蕾的细胞核只出现2C和4C峰，表明组织保持二倍体水平。在花发育过程中一直检测到核内复制。内多倍体可能是甘蓝植株分化过程中常见的现象。这个原始功能的影响进行了讨论。
Flow cytometric analysis of the nuclear DNA contents of somatic tissues from flowers of cabbage (Brassica oleraceaL.) revealed extensive endoreduplication, resulting in tissues that contain cells with multiple ploidy levels (also called ’endopolyploidy’). Multiples of the haploid nuclear genome complement (1C) corresponding to 2C, 4C, 8C, 16C, 32C and 64C were observed in mature flowers. The distribution of cells with the differerent ploidy levels is tissue-specific and is characteristic of the stage of development. Nuclei of young flower buds exclusively gave 2C and 4C peaks, indicating that the tissues maintained diploid level. Endoreduplication was consistently detected during flower development. Endopolyploidy is probably common in differentiation of cabbage plants. Implications of this original feature are discussed.
影响因子：
11.6
作者：
MELARAGNO, JE;MEHROTRA, B;COLEMAN, AW
通讯作者：
COLEMAN, AW