In vitro reaction of endothelial cells to polymer demixed nanotopography
In vitro reaction of endothelial cells to polymer demixed nanotopography
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DOI：
10.1016/s0142-9612(01)00424-0
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发表时间：
2002-07-01
期刊：
影响因子：
14
通讯作者：
Curtis, ASG
中科院分区：
文献类型：
--
作者：
Dalby, MJ;Riehle, MO;Curtis, ASG
引入材料表面的形貌已被证明会强烈影响细胞行为，并且微观表面形貌的影响已被广泛表征。研究现在开始调查细胞对纳米形貌的反应。本研究使用聚苯乙烯和聚（4-溴苯乙烯）的聚合物分层产生纳米高岛，并观察到内皮细胞对岛的反应。研究了三个岛的高度，分别为13、35和95 nm。观察到细胞在制造的形貌上比在类似化学性质的平坦表面上更分散。通过组织学、荧光和扫描电子显微镜也观察到其他形态学差异，在试验表面上观察到许多弓形细胞，并且细胞骨架沿弓形特征沿着。在纳米形貌中，13 nm的岛被认为给出最大的响应，具有高度分散的细胞形态，包含明确的细胞骨架。(C)2002爱思唯尔科技有限公司版权所有。
The introduction of topography to material surfaces has been shown to strongly affect cell behaviour, and the effects of micrometric surface morphologies have been extensively characterised. Research is now starting to investigate the reaction of cells to nanometric topography. This study used polymer demixing of polystyrene and poly(4-bromostyrene) producing nanometrically high islands, and observed endothelial cell response to the islands. Three island heights were investigated; these were 13, 35 and 95 nm. The cells were seen to be more spread on the manufactured topographies than that on flat surfaces of similar chemistry. Other morphological differences were also noted by histology, fluorescence and scanning electron microscopy, with many arcuate cells noted on the test surfaces, and cytoskeletal alignment along the arcuate features. Of the nanotopographies, the 13 nm islands were seen to give the largest response, with highly spread cell morphologies containing well-defined cytoskeleton. (C) 2002 Elsevier Science Ltd. All rights reserved.