Analysis of the 22-NBD-cholesterol transfer between liposome membranes and its relation to the intermembrane exchange of 25-hydroxycholesterol
Analysis of the 22-NBD-cholesterol transfer between liposome membranes and its relation to the intermembrane exchange of 25-hydroxycholesterol
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DOI：
10.1016/j.colsurfb.2010.01.002
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发表时间：
2010-05-01
影响因子：
5.8
通讯作者：
Kuboi, Ryoichi
中科院分区：
文献类型：
--
作者：
Ishii, Haruyuki;Shimanouchi, Toshinori;Kuboi, Ryoichi
采用荧光共振能量转移（FRET）方法定量分析了22- nbd -胆固醇（22-（N-（7-硝基苯-2-氧-1,3-二唑-4-基）氨基）-23,24-双诺-5-胆碱-3-醇）在两种脂质体膜之间的转移。用22- nbd -胆固醇和罗丹明标记的磷脂酰乙醇胺（Rh-DHPE）标记的脂质体作为供体脂质体，监测22- nbd -胆固醇从这些供体脂质体转移到由相同类型的磷脂酰胆碱制备的受体脂质体。结果表明，该反应由快相和慢相组成，所有的动力学测量都可以用双指数模型拟合。结果表明，22- nbd -胆固醇在脂质体膜之间的转移动力学取决于所使用的脂质体的流动性，曲率可能影响动力学。此外，22- nbd -胆固醇在脂膜中的行为类似于氧甾醇25-羟基胆固醇，而不是胆固醇。22- nbd -胆固醇可以作为一种有用的荧光探针来模拟氧化胆固醇如25-羟基胆固醇的膜间转移，而不是胆固醇本身的膜间转移。(C) 2010 Elsevier B.V.版权所有
The transfer of 22-NBD-cholesterol (22-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3-ol) between two liposome membranes was quantitatively analyzed by using the fluorescence resonance energy transfer (FRET) method. Liposomes labeled with both 22-NBD-cholesterol and a rhodamine-labeled phosphatidylethanolamine (Rh-DHPE) were used as donor liposomes, and the 22-NBD-cholesterol transfer from these donor liposomes to acceptor liposomes prepared from same type of phosphatidylcholine was monitored. The transfer kinetics was found to be composed of a fast and a slow phase, and all kinetic measurements could be fitted with a bi-exponential model. The results obtained indicate that the 22-NBD-cholesterol transfer kinetics between liposome membranes depends on the fluidity of the liposome used and that the curvature may affect the kinetics. Furthermore, the behavior of 22-NBD-cholesterol in lipid membrane is similar to that of the oxysterol 25-hydroxycholesterol rather than cholesterol. It is proposed that 22-NBD-cholesterol can be a useful fluorescent probe to mimic the intermembrane transfer of oxidized cholesterols like 25-hydroxycholesterol, rather than that of cholesterol itself. (C) 2010 Elsevier B.V. All rights reserved.