GLUT-4 AND INSULIN-RECEPTOR BINDING AND KINASE-ACTIVITY IN TRAINED HUMAN MUSCLE
GLUT-4 AND INSULIN-RECEPTOR BINDING AND KINASE-ACTIVITY IN TRAINED HUMAN MUSCLE
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DOI：
10.1113/jphysiol.1993.sp019833
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发表时间：
1993-09-01
影响因子：
5.5
通讯作者：
GALBO, H
中科院分区：
文献类型：
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作者：
DELA, F;HANDBERG, A;GALBO, H
1.体育锻炼可增强胰岛素介导的人体肌肉葡萄糖摄取的敏感性和反应性。本研究探讨了体育锻炼的这种效果是由于胰岛素受体功能的增加或胰岛素可募集葡萄糖转运蛋白（GLUT 4）总浓度的增加. 7名健康青年受试者在单腿最大摄氧量（V（O2，max））的70%下进行了10周的单腿自行车训练。随后从两条腿的股外侧肌进行活检。训练腿的单腿V（O2，最大值）增加（46+/-3至52+/-2 ml min-1 kg-1（平均值+/-S.E. M.，P < 0.05），细胞色素c氧化酶活性高于未训练的腿（2.0+/-0.1 vs. 1.4+/-0.1 nmol s-1（mg肌肉）-1，P < 0.05）。胰岛素结合以及基础和胰岛素刺激的受体激酶活性在训练和未训练的肌肉之间没有差异。前者GLUT 4蛋白浓度较高（14.9+/-1.9 vs. 11.6+/-1.0任意单位（mug蛋白）-1）。训练诱导的GLUT 4增加（26+/-11%）与先前报道的相同受试者中胰岛素刺激的最大腿部葡萄糖摄取增加（25+/-7%）相匹配，两个变量的个体值相关（相关系数（r）= 0.84，P < 0.05）。总之，在人类肌肉训练中，诱导GLUT 4蛋白的局部收缩依赖性增加，这增强了胰岛素对葡萄糖摄取的作用。另一方面，肌肉中的胰岛素受体功能不太可能受到训练的影响。
1. Physical training enhances sensitivity and responsiveness of insulin-mediated glucose uptake in human muscle. This study examines it this effect of physical training is due to increased insulin receptor function or increased total concentration of insulin-recruitable glucose transporter protein (GLUT 4).2. Seven healthy young subjects carried out single leg bicycle training for 10 weeks at 70% of one leg maximal oxygen uptake (V(O2,max)). Subsequently biopsies were taken from the vastus lateralis muscle of both legs.3. Single leg V(O2,max) increased for the trained leg (46+/-3 to 52+/-2 ml min-1 kg-1 (means+/-S.E. M., P < 0.05), and cytochrome c oxidase activity was higher in this compared to the untrained leg (2.0+/-0.1 vs. 1.4+/-0.1 nmol s-1 (mg muscle)-1, P < 0.05). Insulin binding as well as basal- and insulin-stimulated receptor kinase activity did not differ between trained and untrained muscle. The concentration of GLUT 4 protein was higher in the former (14.9+/-1.9 vs. 11.6+/-1.0 arbitrary units (mug protein)-1 in crude membranes, P < 0.05). The training-induced increase in GLUT 4 (26+/-11%) matched a previously reported increase in maximum insulin-stimulated leg glucose uptake (25+/-7%) in the same subjects, and individual values of the two variables correlated (correlation coefficient (r) = 0.84, P < 0.05).4. In conclusion, in human muscle training induces a local contraction- dependent increase in GLUT 4 protein, which enhances the effect of insulin on glucose uptake. On the other hand, insulin receptor function in muscle is unlikely to be affected by training.