Stamp Wound Assay for Studying Coupled Cell Migration and Cell Debris Clearance
Stamp Wound Assay for Studying Coupled Cell Migration and Cell Debris Clearance
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DOI：
10.1021/la103542y
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发表时间：
2010-11-16
期刊：
影响因子：
3.9
通讯作者：
Lele, Tanmay P.
中科院分区：
文献类型：
--
作者：
Lee, Jiyeon;Wang, Yu-Lin;Lele, Tanmay P.
建立了一种在体外真实条件下研究创伤愈合的新方法。该方法涉及用聚（二甲基）硅氧烷（PDMS）冲压产生受损细胞碎片的限定图案。这种新的测定允许在存在细胞碎片的情况下定量伤口愈合速率。该试验的实验结果表明，在存在细胞碎片的情况下，细胞迁移是一个两步过程，需要（1）非肌肉肌球蛋白II依赖性细胞清除，然后（2）细胞迁移到新清除的伤口区域。新的戳伤口测定允许研究偶联的细胞迁移和碎片清除，并且是更现实的体外伤口愈合测定。
A new method for studying wound healing under realistic conditions in vitro was developed. The method involves creating defined patterns of damaged cell debris with poly(dimethyl)siloxane (PDMS) stamping. This novel assay permitted the quantification of wound healing rates in the presence or cell debris. Experimental results with this assay suggest that cell migration in the presence of cell debris is a two step process requiring (1) non-muscle myosin II-dependent cell clearance followed by (2) cell migration into newly cleared wound areas. The novel stamp wound assay allows the study of coupled cell migration and debris clearance and is a more realistic wound healing assay in vitro.