Validation of different methods to compare isovolumic cardiac function in isolated hearts of varying sizes.
Validation of different methods to compare isovolumic cardiac function in isolated hearts of varying sizes.
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验证不同方法比较不同大小的离体心脏的等容心脏功能。
DOI：
10.1152/ajpheart.1997.272.1.h501
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Morgan,JP
中科院分区：
文献类型：
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作者：
Strömer,H;Cittadini,A;Szymanska,G;Apstein,CS;Morgan,JP
不同大小的离体心脏的功能比较一直很困难，因为不同心室容量的功能不同。到目前为止，还没有建立标准体积。为了确定最准确的实验方法，我们测试了五种不同的方法来标准化大小不同但乳头肌功能相似的对照心脏和同心肥厚心脏的体积：心内球囊体积(VB) = 120微升（M1）。舒张压时的VB = 10 mmHg （M2）或舒张壁应力= 4 kdyn/cm2 (M3), V1 = 25微升/100 g体重（M4）， VB =峰值发育压力时体积的50% （Vmax; M5）。对照组的收缩期和舒张期功能在M1组有差异，在M2或M5组有可比性。M3和M4表现为临界显著性差异。我们认为M5和M2适用于不同大小心脏的功能比较。如果对舒张顺应性感兴趣，如同心性肥厚，则参数-体积曲线应通过Vmax归一化，以比较Frank-Starling曲线对应点的功能（例如，在Vmax的50%，M5）。
Functional comparison of isolated hearts with different sizes has been difficult because function varies at different ventricular volumes. To date, no standard volume has been established. To determine the most accurate experimental approach, we tested five different methods to standardize volume in control hearts with different sizes but similar papillary muscle function and in hearts with concentric hypertrophy: intracardiac balloon volume (VB) = 120 microliters (M1). VB at diastolic pressure = 10 mmHg (M2) or diastolic wall stress = 4 kdyn/cm2 (M3), V1 = 25 microliters/100 g body weight (M4) and VB = 50% of volume at peak developed pressure (Vmax; M5). Systolic and diastolic functions of control groups were different using M1 and comparable using M2 or M5. M3 and M4 showed borderline significant differences. We concluded that M5 and M2 were suitable to compare function among hearts of different sizes. If diastolic compliance is of interest, as in concentric hypertrophy, parameter-volume curves should be normalized by Vmax to compare function at corresponding points of the Frank-Starling curve (e.g., at 50% of Vmax, M5).